1. Expression of microRNAs during chondrogenesis of human adipose-derived stem cells 
Z. Zhang, Y. Kang, Z. Zhang, H. Zhang, X. Duan, J. Liu, X. Li, W. Liao 
Osteoarthritis and Cartilage 
Volume 20, Issue 12, Pages (December 2012)
DOI: /j.joca
Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

2. Fig. 1
Morphology of hADSC cells after chondrogenic induction (100×). Cells at passage 3 were cultured as micromasses and induced for chondrogenic differentiation using inducing buffer. After 21 days, the micromasses with hADSCs were stained with Alcian blue and visualized under microscope. (A) Control hADSC cells without induction for chondrogenic differentiation at day 6. (B) hADSC cells after induction for chondrogenic differentiation by micromass culture at day 21.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

3. Fig. 2
Determination of chondrogenesis-related proteins expression to confirm chondrogenic differentiation. hADSC cells were induced for chondrogenic differentiation. At day 0, 6, 10 and 14, media were collected and chondrogenesis-related protein concentrations of Col2a1, Col10a1, aggrecan and chondroitin were detected using ELISA. Basal level was obtained using parallel growth media without chondrogenic induction at same time point. Mean ± S.D. values (n = 3 cultures) were presented on the graph. Pair t-test was implemented for comparisons between chondrogenic and basal group at a given time point (* indicates P < 0.05; ** means P < 0.005; *** denotes P < 0.001).
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

4. Fig. 3
qRT-PCR confirmation of miRNA expression in chondrogenic differentiated hADSC cells. 1 μg of total RNA purified from undifferentiated and differentiated hADSCs were used for qRT-PCR analysis. The RNA samples were reverse transcribed to cDNA, and then amplified. Quantitative real-time PCR was done with indicated primers and fold-change of mRNA was normalized to U6 mRNA. Each point represents the mean value of fold change compared with undifferentiated cell control samples (set as 1) from three patients, and each was repeated three times.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

5. Fig. 4
The expression of targeting transcription factors in chondrogenic differentiated hADSC cells. RNAs from undifferentiated and differentiated hADSCs were tested. Quantitative real-time PCR was done with indicated primers and fold-change of mRNA was normalized to U6 mRNA. Each bar represents the mean ± S.D. of fold change compared with undifferentiated cell control samples (set as 1) from three patients, and each was repeated three times (*** indicates P < 0.001).
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions