1. Dihybrid Cross

2. Dihybrid Cross
Aladdin and Jasmine want to have a child. Aladdin is homozygous for brown hair and brown eyes. Jasmine is homozygous for black and black eyes. Perform a test cross and predict the possible genotypes and phenotypes for their offspring. (B- Black hair; b-brown hair) (E- black eyes; e- brown eyes)

3. Dihybrid Cross Step 1- Create a Key B: black hair b: brown hair
E: black eyes
e: brown eyes

4. Dihybrid Cross Step 2: Identify the parent genotypes
Aladdin: homozygous for brown hair and eye
_________
Jasmine: homozygous for black hair and eyes

5. Dihybrid Cross Step 2a- Number the parent alelles: Aladdin: b1b2e3e4
bbee x BBEE
Aladdin: b1b2e3e4
Jasmine: B1B2E3E4
Aladin:
1-3 _______
1-4 _______
2-3 _______
2-4 _______
Jasmine:
1-3 ______
1-4 ______
2-3 ______
2-4 ______

6. Dihybrid Cross Step 3a: Labeling the punnett square
Aladin is on top of the box
be be be be
Aladin:
1-3 (be)
1-4 (be)
2-3 (be)
2-4 (be)

7. Dihybrid Cross Step 3b: label the side of the punnett square
Jasmine : BBEE be be be be BE
Jasmine:
1-3 (BE)
1-4 (BE)
2-3 (BE)
2-4 (BE)

8. Dihybrid Cross
Step 4: Fill in all 16 boxes with the possible gametes from the cross
Each box will have 4 letters or 2 traits
be be be be
Aladin:
1-3 (be)
1-4 (be)
2-3 (be)
2-4 (be)
(BE)
Jasmine:
1-3 (BE)
1-4 (BE)
2-3 (BE)
2-4 (BE)

9. Dihybrid Cross
Step 5: List all possible genotypes and phenotype ratios
be be be be BE
BEbe, BEbe, BEbe , BEbe, BEbe, BEbe, BEbe, BEbe, BEbe, BEbe, BEbe, BEbe, BEbe, BEbe, BEbe ( 100% BEbe)
BEbe

10. Dihybrid Cross Conclusion
All 16 traits were BEbe, which is Heterozygous for Black Hair and Black eyes
Genotype: ______________
Phenotype: ______________________
Happily Ever After
The End