1. Runt en
AMG
PMG

2. Ectopic engrailed eliminates runt in anterior cells
stg
wrapper
wildtype
runt
stg
odd
Stg uas-en
runt

3. Ectopic runt reduces engrailed in most posterior midline cells
wildtype
Stg uas-runt en
stg
CG8965

4. enA/enE mutants ectopically express runt in posterior midline glia
Wildtype
stage 11
stg
wrapper
runt
enA/enE
stage 12
Wrapper is also increased in posterior midline glia in an en- background

5. In anterior midline glia
runt en en
runt
wrapper
AMG
PMG

6. CG32030 is somewhat restricted to AMG at stage 12 and 13
stg
Stage 11
CG32030
Stage 13
Stage 15 en

7. CG32030 levels are similar in PMG and AMG in stg uas-en

8. CG32030 levels are similar in PMG and AMG in stg uas-runt

9. Model
UAS-Hh
en- Hh En
High wrapper
UAS-En
UAS-En
UAS-Runt
en-
UAS-Hh
CG32030
UAS-Runt
Runt