1. FRET change in SCORE precedes fusion.
FRET change in SCORE precedes fusion. (A, I) Sequence of excised FRET ratio images averaged from 903 non-AFS events like that in Fig.1C. (A, II) SCORE fluorescence (sum of CFP and Venus) averaged as in A, I. (B) Average amperometric spike obtained after temporal alignment of the spikes at their starting times. Average time course of (C) Venus and CFP emission and (D) FRET ratio measured at release sites as the red square in Fig. 1 (bold traces) and from control footprint areas (as the cyan circle in Fig. 1 A, II) over the same time periods (faint traces). (E) FRET time course from the data of Fig. 2D analyzed with the ECOM method on expanded time scale (black), fits of the FRET rising phase (−0.6 s to +0.4 s) with the ECOM step response function (red), and with convolution of step response with exponential distribution of 128-ms time constant (blue); averaged ECD spike (green). (F) Squared deviations of the two fits, colors as in E.
Zhao Y et al. PNAS 2013;110:
©2013 by National Academy of Sciences