1. Volume 5, Issue 2, Pages 366-375 (March 2012)
Catabolism of Branched Chain Amino Acids Supports Respiration but Not Volatile Synthesis in Tomato Fruits 
Andrej Kochevenko, Wagner L. Araújo, Gregory S. Maloney, Denise M. Tieman, Phuc Thi Do, Mark G. Taylor, Harry J. Klee, Alisdair R. Fernie 
Molecular Plant 
Volume 5, Issue 2, Pages (March 2012)
DOI: /mp/ssr108
Copyright © 2012 The Authors. All rights reserved. Terms and Conditions

2. Figure 1
Branched-Chain Amino Acid Biosynthetic and Degradation Pathways in Plants.
(A) The pathway with key enzymes (red circles) and metabolites (green) of branched-chain amino acid biosynthesis are shown. (1) threonine deaminase, (2) acetolactate synthase, (3) acetolactate isomeroreductase, (4) dihydroxy-acid dehydratase, (5) branched-chain aminotransferase, (6) 2-isopropylmalate synthase, (7) isopropylmalate isomerase, (8) isopropylmalate dehydrogenase.
(B) The pathway with key enzymes (blue) and metabolites (green) of branched-chain amino acid degradation is shown.
Molecular Plant 2012 5, DOI: ( /mp/ssr108)
Copyright © 2012 The Authors. All rights reserved. Terms and Conditions

3. Figure 2
Volatile Production Following Branched-Chain Substrate Feeding in Tomato Pericarp Discs.
Graphs show the changes in branched-chain volatile compounds after feeding of tomato pericarp discs with the BCAA or BCKA substrate specified above each graph after 6 h of incubation. Values are expressed in percentage of volatile emission of water-fed controls ± SE. Columns marked with an asterisk indicate statistically significant changes, as determined by Student’s t-test (P < 0.05).
Molecular Plant 2012 5, DOI: ( /mp/ssr108)
Copyright © 2012 The Authors. All rights reserved. Terms and Conditions

4. Figure 3
Respiration Rate in Pericarp Discs of Wild-Type and SlBCAT1 Antisense Plants.
Comparative analyses of respiration in fruit pericarp tissue of different ages. O2 consumption (nmol min−1 g−1 FW) of green (A) and red ripe (B) fruits. Graphs show the rate of oxygen consumption as measured in a Clark-type electrode. Values represent the mean ± SE of six independent determinations. Columns marked with an asterisk indicate statistically significant changes, as determined by Student’s t-test (P < 0.05).
Molecular Plant 2012 5, DOI: ( /mp/ssr108)
Copyright © 2012 The Authors. All rights reserved. Terms and Conditions

5. Figure 4
Pathways of Branched Chain Amino Acid Metabolism. A Proposed biosynthetic pathways for Branched Chain Amino Acid Degradation to Volatiles in Tomato Plants.
Red route: decarboxylation first followed by deamination with an amine intermediate, catalyzed by amino acid decarboxylases and deaminases, as partially demonstrated in tomato fruit (Tieman et al., 2006a). Black route: transamination followed by decarboxylation with an α-keto acid intermediate, catalyzed by two separate enzymes, as partially demonstrated in this work and in the work of Gonda and co-workers (2010). The size of an arrow indicates its contribution to the final production of volatiles. Solid arrows indicate that the enzyme activity and genes have been identified. Dashed arrows indicate that the proposed plant enzymes and genes are yet to be identified B. Steps that constitute the described pathway of branched-chain volatiles from BCAAs in microbes.
Molecular Plant 2012 5, DOI: ( /mp/ssr108)
Copyright © 2012 The Authors. All rights reserved. Terms and Conditions