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E1B-55 kDa-Defective Adenoviruses Activate p53 in Mesothelioma and Enhance Cytotoxicity of Anticancer Agents  Makako Yamanaka, MD, Yuji Tada, MD, PhD,

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Presentation on theme: "E1B-55 kDa-Defective Adenoviruses Activate p53 in Mesothelioma and Enhance Cytotoxicity of Anticancer Agents  Makako Yamanaka, MD, Yuji Tada, MD, PhD,"— Presentation transcript:

1 E1B-55 kDa-Defective Adenoviruses Activate p53 in Mesothelioma and Enhance Cytotoxicity of Anticancer Agents  Makako Yamanaka, MD, Yuji Tada, MD, PhD, Kiyoko Kawamura, BS, Quanhai Li, PhD, Shinya Okamoto, PhD, Kuan Chai, BS, Sana Yokoi, MD, PhD, Min Liang, PhD, Toshihiko Fukamachi, PhD, Hiroshi Kobayashi, PhD, Naoto Yamaguchi, PhD, Atsushi Kitamura, MD, Hideaki Shimada, MD, PhD, Kenzo Hiroshima, MD, PhD, Yuichi Takiguchi, MD, PhD, Koichiro Tatsumi, MD, PhD, Masatoshi Tagawa, MD, PhD  Journal of Thoracic Oncology  Volume 7, Issue 12, Pages (December 2012) DOI: /JTO.0b013e fa4 Copyright © 2012 International Association for the Study of Lung Cancer Terms and Conditions

2 FIGURE 1 A, Cytotoxicity of Ad-delE1B55. Cells were infected with Ad-delE1B55 or Ad-LacZ, and the relative cell viability was calculated based on values of uninfected cells as 100%. SEs and IC50 (vp/cell) of Ad-delE1B55 are also shown. B, Expression levels of CAR molecules analyzed with flow cytometry. The bar values showed mean fluorescence intensity expressed with an arbitrary FL1 unit. HEK293 cells were used for a standard. Journal of Thoracic Oncology 2012 7, DOI: ( /JTO.0b013e fa4) Copyright © 2012 International Association for the Study of Lung Cancer Terms and Conditions

3 FIGURE 2 A, Cell proliferation and (B), viability of Ad-delE1B55–infected cells. NCI-H28 and MSTO-211H cells were infected with Ad (NCI-H28: 1 × 103, MSTO-211H: 4.5 × 103 vp/cell), and the cell numbers and the viability were determined with the trypan blue dye exclusion test. Cells reached at the confluence were seeded to keep cell growing. SEs are shown. *p < 0.01, compared with no treatment or Ad-LacZ group. Journal of Thoracic Oncology 2012 7, DOI: ( /JTO.0b013e fa4) Copyright © 2012 International Association for the Study of Lung Cancer Terms and Conditions

4 FIGURE 3 A and B, Ad-delE1B55–induced activation of p53 pathways. NCI-H28 and (C), MSTO-211H, cells were infected with Ad-delE1B55 (del-E1B) or Ad-LacZ (Ad-Z) at 1.5 × 103 vp/cell and cultured for the indicated times. Expression levels were analyzed with Western blot analyses. Actin was used as a control. Journal of Thoracic Oncology 2012 7, DOI: ( /JTO.0b013e fa4) Copyright © 2012 International Association for the Study of Lung Cancer Terms and Conditions

5 FIGURE 4 Representative profiles of cell-cycle analyses. Cells were treated with Ad-delE1B55 or Ad-LacZ (NCI-H28: 1 × 103, MSTO-211H: 4.5 × 103 vp/cell), or untreated for 72 hours. Journal of Thoracic Oncology 2012 7, DOI: ( /JTO.0b013e fa4) Copyright © 2012 International Association for the Study of Lung Cancer Terms and Conditions

6 FIGURE 5 A, Nuclear morphological changes induced by Ad-delE1B55. NCI-H28 cells were infected with Ad (1 × 103 vp/cell) followed by the Giemsa staining. In Ad-delE1B55–treated cells, small and highly condensed nuclei (solid arrow) and large and uncondensed nuclei (open) were detected. B, NCI-H28 cells infected with Ad (1 × 103 vp/cell) were examined for apoptosis with the TUNEL assay and stained with Kernechtrot solution to visualize nuclei. TUNEL, terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate-biotin nick end-labeling. Journal of Thoracic Oncology 2012 7, DOI: ( /JTO.0b013e fa4) Copyright © 2012 International Association for the Study of Lung Cancer Terms and Conditions

7 FIGURE 6 A, Combinatory effects of Ad-delE1B55 and CDDP or PEM. Cells were treated with different doses of CDDP or (C) PEM, and the relative cell viabilities were calculated based on values of untreated cells as 100%. B, Cells were also treated with various vp of Ad-delE1B55 and concentrations of CDDP or (D), PEM. CIs were calculated with CalcuSyn software and plotted in respective Fa points. SEs are shown. E, MSTO-211H cells were treated with Ad-delE1B55 (1.5 × 103 vp/cell) and/or with CDDP (10 μM). Expression levels of the indicated proteins were analyzed with Western blot analyses. Actin was used as a control. CDDP, cis-diamminedichloroplatinum; PEM, pemetrexed. Journal of Thoracic Oncology 2012 7, DOI: ( /JTO.0b013e fa4) Copyright © 2012 International Association for the Study of Lung Cancer Terms and Conditions

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