1. Two-Photon Luminescent Bone Imaging Using Europium Nanoagents
Esther M. Surender, Steve Comby, Brenton L. Cavanagh, Orlaith Brennan, T. Clive Lee, Thorfinnur Gunnlaugsson 
Chem 
Volume 1, Issue 3, Pages (September 2016)
DOI: /j.chempr
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2. Chem 2016 1, DOI: ( /j.chempr )
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3. Figure 1 Structural Design of the Nanoparticulate Imaging Agent
(A) The 1,3-diketone-sensitizing ligand nta.
(B) The synthesized complex 1.Eu.Na.
(C) The surface-functionalized AuNP system AuNP-1.Eu.Na.
Chem 2016 1, DOI: ( /j.chempr )
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4. Figure 2
DLS and TEM Characterization of the Size of Functionalized Gold Nanoparticles
(A) Volume-distribution profile determined by DLS analysis for AuNP-1.Eu.Na (1 × 10−7 M) in H2O at 298 K.
(B) Size-distribution profile calculated from analysis of the TEM images of AuNP-1.Eu.Na (4 × 10−7 M).
Chem 2016 1, DOI: ( /j.chempr )
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5. Figure 3 Spectroscopic Determination of 1:1 Ternary-Complex Formation
Changes in the normalized Eu(III) emission (λex = 330 nm) of 1.Eu.Na (10 μM, ) and AuNP-1.Eu.Na (0.1 μM) at 612 and 616 nm, respectively, as a function of equivalents of nta in buffered 0.1 M HEPES solution at pH 7.4 (I = 0.1 M NaCl) () and D2O ().
Chem 2016 1, DOI: ( /j.chempr )
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6. Figure 4
Phosphorescence Studies Showing the pH Dependence of the Ternary Complex System AuNP-1.Eu.Na-nta in Aqueous Solution
(A) The phosphorescent response of AuNP-1.Eu.Na (1.0 × 10−7 M) and nta (20 equiv) as a function of pH in H2O (I = 0.1) at 298 K (λexc = 330 nm).
(B) Changes in the integrated Eu(III) emission as a function of pH for both the forward (pH 9.0→3.5, red) and backward (pH 3.5→9.0, blue) titrations, measured between 570 and 720 nm.
Chem 2016 1, DOI: ( /j.chempr )
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7. Figure 5 Illustration of Microscratched Bovine Bone Specimens
Specimens were immersed in either AuNP-1.Eu.Na or AuNP-1.Eu initially for 20 hr and then for a further 4 hr.
Chem 2016 1, DOI: ( /j.chempr )
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8. Figure 6 Microscopy of Microdamaged Bovine Bone Structure
Images show microdamaged bovine bone structure after being immersed for 0, 4, and 24 hr in an aqueous solution of AuNP-1.Eu.Na (3.1 × 10−4 M) and then immersed in an aqueous solution of nta (1.5 × 10−4 M).
(A) Polarized light images.
(B) Epifluorescence images were acquired from a LED source (λexc = 365 nm) and a 520 longpass emission filter.
All scale bars represent 250 μm; aqueous solution = buffered 0.1 M HEPES (I = 0.1 M NaCl [pH 7.4]).
Chem 2016 1, DOI: ( /j.chempr )
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9. Figure 7
TPE Fluorescence Microscopy of Microdamaged Bovine Bone Structure
Images show microdamaged bovine bone structure after being immersed for 0, 4, and 24 hr in an aqueous solution of AuNP-1.Eu.Na (7.8 × 10−5 M) and then immersed in an aqueous solution of nta (1.5 × 10−4 M) for 30 s.
(A) Images taken at the bottom of the microcrack.
(B) Images taken at the top of the microcrack.
(C) Whole-projection images of the microcrack (z stack).
Each image was acquired with a Ti-sapphire laser (λ = 750 nm) and a red-channel emission filter (λem = 565–610 nm). All scale bars represent 150 μm; aqueous solution = buffered 0.1 M HEPES (I = 0.1 M NaCl [pH 7.4]).
Chem 2016 1, DOI: ( /j.chempr )
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10. Figure 8 2D TPE Microscopy of Microdamaged Bovine Bone Structure
Images show microdamaged bovine bone structure after being immersed for 0, 4, and 24 hr in an aqueous solution of AuNP-1.Eu.Na and then immersed in an aqueous solution of nta (1.5 × 10−4 M) for 30 s. All scale bars represent 85 μm.
(A) Concentration of AuNP-1.Eu.Na = 3.1 × 10−4 M.
(B) Concentration of AuNP-1.Eu.Na = 1.6 × 10−4 M.
Chem 2016 1, DOI: ( /j.chempr )
Copyright © 2016 Elsevier Inc. Terms and Conditions

11. Figure 9 3D TPE Microscopy of Microdamaged Bovine Bone Structure
Images show microdamaged bovine bone structure after being immersed for 0, 4, and 24 hr in an aqueous solution of AuNP-1.Eu.Na and then immersed in an aqueous solution of nta (1.5 × 10−4 M) for 30 s. All scale bars represent 85 μm.
(A) Concentration of AuNP-1.Eu.Na = 3.1 × 10−4 M.
(B) Concentration of AuNP-1.Eu.Na = 1.6 × 10−4 M.
Chem 2016 1, DOI: ( /j.chempr )
Copyright © 2016 Elsevier Inc. Terms and Conditions