1. Human TSLP promotes CD40 ligand-induced IL-12 production by myeloid dendritic cells but maintains their Th2 priming potential
by Norihiko Watanabe, Shino Hanabuchi, Marie-Annick Marloie-Provost, Svetlana Antonenko, Yong-Jun Liu, and Vassili Soumelis
Blood
Volume 105(12):
June 15, 2005
©2005 by American Society of Hematology

2. TSLP enhances CD40L-induced bioactive IL-12p70 production by CD11c+ DCs. (A) Blood CD11c+ DCs were activated for 24 hours in different conditions, and IL-12p70 was measured in the culture supernatant by high-sensitivity protein ELISA. Each triangle represen...
TSLP enhances CD40L-induced bioactive IL-12p70 production by CD11c+ DCs. (A) Blood CD11c+ DCs were activated for 24 hours in different conditions, and IL-12p70 was measured in the culture supernatant by high-sensitivity protein ELISA. Each triangle represents IL-12p70 production by DCs in a different donor. (B-C) CD11c+ DCs were first primed for 24 hours in different conditions, and IL-12p70 was measured by protein ELISA in this first set of supernatants (0-24 hours; □). After extensive washing of the DCs, equal numbers of cells from each initial condition were restimulated for 24 hours, and IL-12p70 was measured in the culture supernatant (24-48 hours; ▪). (B) Priming for 24 hours with medium alone, TSLP, CD40L, or TSLP+CD40L and subsequent 24-hour restimulation with CD40L for all culture conditions. (C) Priming for 24 hours with IL-4 or TSLP (0-24 hours; no IL-12 production) followed by 24-hour restimulation with CD40L, lipopolysaccharide (LPS), or poly(I:C) (24-48 hours). (D) CD11c+ DCs were primed for 24 hours with IL-4, TSLP, CD40L, or a combination of these conditions. IL-12p70 and IL-12p40 were measured in the culture supernatant by protein ELISA. Data shown are from 1 of 3 representative independent experiments.
Norihiko Watanabe et al. Blood 2005;105:
©2005 by American Society of Hematology

3. CD11c+ DCs activated by TSLP together with CD40L prime naive CD4+ T cells to produce Th1 and Th2 cytokines.
CD11c+ DCs activated by TSLP together with CD40L prime naive CD4+ T cells to produce Th1 and Th2 cytokines. (A) DCs activated with TSLP, CD40L, or both stimuli were used to prime naive CD4+ T cells. After 6 days of coculture, T cells were restimulated for 24 hours with anti-CD3 and anti-CD28, and T cell-derived cytokines were measured in the culture supernatant using ELISA. (B) DCs activated by TSLP or IL-4 with or without CD40L were used to prime naive CD4+ T cells. After 6 days of coculture, T cells were restimulated for 5 hours with PMA and ionomycin, and T cell-derived cytokine production was determined by intracellular cytokine staining. Numbers indicate the percent of cells in each quadrant. Data shown are from 1 of 3 representative independent experiments.
Norihiko Watanabe et al. Blood 2005;105:
©2005 by American Society of Hematology