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Comparison of Snap Freezing versus Ethanol Fixation for Gene Expression Profiling of Tissue Specimens  Mark A. Perlmutter, Carolyn J.M. Best, John W.

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Presentation on theme: "Comparison of Snap Freezing versus Ethanol Fixation for Gene Expression Profiling of Tissue Specimens  Mark A. Perlmutter, Carolyn J.M. Best, John W."— Presentation transcript:

1 Comparison of Snap Freezing versus Ethanol Fixation for Gene Expression Profiling of Tissue Specimens  Mark A. Perlmutter, Carolyn J.M. Best, John W. Gillespie, Yvonne Gathright, Sergio González, Alfredo Velasco, W. Marston Linehan, Michael R. Emmert-Buck, Rodrigo F. Chuaqui  The Journal of Molecular Diagnostics  Volume 6, Issue 4, Pages (November 2004) DOI: /S (10)60534-X Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Total RNA after microdissection, before amplification. Lanes 1 to 4, frozen samples. Lane 5, ethanol-paraffin sample. The Journal of Molecular Diagnostics 2004 6, DOI: ( /S (10)60534-X) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Hierarchical clustering of arrays using all probe sets. A: Hierarchical clustering of all arrays. B: Hierarchical clustering of ethanol-paraffin samples. E-P, ethanol-paraffin; Fr, frozen; BPH, benign prostatic hyperplasia; Tu: tumor. The Journal of Molecular Diagnostics 2004 6, DOI: ( /S (10)60534-X) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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