1. Analysis of a Genetic Polymorphism in the Costimulatory Molecule TNFSF4 with Hematopoietic Stem Cell Transplant Outcomes 
Peter T. Jindra, Susan E. Conway, Stacy M. Ricklefs, Stephen F. Porcella, Sarah L. Anzick, Mike Haagenson, Tao Wang, Stephen Spellman, Edgar Milford, Peter Kraft, David H. McDermott, Reza Abdi 
Biology of Blood and Marrow Transplantation 
Volume 22, Issue 1, Pages (January 2016)
DOI: /j.bbmt
Copyright © 2016 American Society for Blood and Marrow Transplantation Terms and Conditions

2. Figure 1
Donor TNFSF4 rs C/C genotype versus C/T and T/T in a discovery cohort. Kaplan-Meier curves for (A) DFS, (B) patient OS, (C) cumulative incidence of TRM, and (D) cumulative incidence of relapse in the 10 years after transplantation. (E) Cumulative incidence curves for aGVHD grades II to IV (E) and III to IV (F) in the first 100 days after transplantation.
Biology of Blood and Marrow Transplantation  , 27-36DOI: ( /j.bbmt )
Copyright © 2016 American Society for Blood and Marrow Transplantation Terms and Conditions

3. Figure 2
TNFSF4 gene organization as depicted in the NCBI database and analysis of SNPs in linkage with rs The TNFSF4 gene is on chromosome 1q25 and is a 3-exon, 2-intron gene. Filled boxes denote exons, unfilled boxes indicate untranslated regions, and solid lines between boxes represent introns in the TNFSF4 gene diagram. The relative positions of the TNFSF4 variants tagged with rs are indicated along with their percent of linkage disequilibrium calculated in Haploview. A haplotype frequency is noted for the linked major alleles compared with minor alleles. The assessment of each SNPs functional relevance was calculated using F-SNP bioinfomatics algorithm. Potential transcription factor binding sites altered by the SNP polymorphism are listed.
Biology of Blood and Marrow Transplantation  , 27-36DOI: ( /j.bbmt )
Copyright © 2016 American Society for Blood and Marrow Transplantation Terms and Conditions

4. Figure 3
Specific binding of c-myb to a 30-oligomer sequence of TNFSF4 containing the rs polymorphism. (A) Binding of c-myb to a 30-mer oligonucleotide probe containing either the rs C or T variant in the center. Binding was measured as a function of absorbance at 450 nm after a peroxidase reaction. Data represent 3 independent experiments. (B) The specific binding of c-myb to the rs C probe was shown by a competition experiment with nonbiotinylated dsDNA. Different concentrations of probe (100, 1000 pmol) were added to Jurkat nuclear extract and incubated on an ELISA plate coated with 2 pmol of biotinylated rs C probe and absorbance read at 450 nm after a peroxidase reaction. Data represent 2 independent experiments. B indicates blank; Bio, biotinylated; c-myb, myeloblastosis viral oncogene homolog.
Biology of Blood and Marrow Transplantation  , 27-36DOI: ( /j.bbmt )
Copyright © 2016 American Society for Blood and Marrow Transplantation Terms and Conditions

5. Figure 4
TNFSF4 surface expression on CD19+ B cells from HSCT donor peripheral blood mononuclear cells. Peripheral blood mononuclear cells (n = 12) from each TNFSF4 rs genotype (n = 4) were stimulated with 10 μg/mL IgM and 5 μg/mL anti-CD40 antibodies for 72 hours. (A) Overlaid histograms were normalized and depicted as the % of max for the CC (i), TC (ii), and TT (iii) genotypes. Black histograms indicate TNFSF4 staining of stimulated B cells, and the gray histograms indicate TNFSF4 staining of unstimulated B cells. A negative baseline cutoff was created based on isotype staining demarcated where the negative and positive histogram markers overlap. (B) The percentage of TNFSF4 positive B cells was calculated in the presence and absence of stimulation. The average difference in the percentage of TNFSF4 positive B cells with and without stimulation for each genotype was plotted with standard error of mean.
Biology of Blood and Marrow Transplantation  , 27-36DOI: ( /j.bbmt )
Copyright © 2016 American Society for Blood and Marrow Transplantation Terms and Conditions