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Volume 132, Issue 1, Pages (January 2007)

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1 Volume 132, Issue 1, Pages 45-51 (January 2007)
Chronic Intestinal Pseudo-Obstruction due to Buserelin-Induced Formation of Anti- GnRH Antibodies  Bodil Ohlsson, Béla Veress, Sabina Janciauskiene, Agneta Montgomery, Monica Haglund, Anders Wallmark  Gastroenterology  Volume 132, Issue 1, Pages (January 2007) DOI: /j.gastro Copyright © 2007 AGA Institute Terms and Conditions

2 Figure 1 (A) Myenteric ganglion with both swollen neurons containing vacuoles within the cytoplasm (arrows) and shrunken neurons with pyknotic nuclei (arrowheads); the bottom cell of these cells is also vacuolated. (H&E, original magnification ×320.) (B) The swollen neuron is negative for PGP9.5 (arrow; peroxidase–antiperoxidase immunohistochemistry for neuron-specific PGP9.5; original magnification ×320). Gastroenterology  , 45-51DOI: ( /j.gastro ) Copyright © 2007 AGA Institute Terms and Conditions

3 Figure 2 (A) Inner part of circular muscle layer with numerous ICCs; a few of them contain vacuoles in the cytoplasm (arrows). (B) Perimyenteric ICC-plexus with cytoplasmic vacuoles (arrows). (Peroxidase–antiperoxidase immunohistochemistry for c-kit/CD117; original magnification ×280.) Gastroenterology  , 45-51DOI: ( /j.gastro ) Copyright © 2007 AGA Institute Terms and Conditions

4 Figure 3 (A) Control myenteric ganglion with eight neurons; several of these are immunoreactive for GnRH. The arrow shows one of several positive neurons. (B) Myenteric ganglion from the patient with three neurons; the two larger ones are negative to the GnRH antibody, whereas there are a few immunostained granules within the cytoplasm of a moderately shrunken neuron (arrow). (Peroxidase–antiperoxidase for GnRH; original magnification ×420.) Gastroenterology  , 45-51DOI: ( /j.gastro ) Copyright © 2007 AGA Institute Terms and Conditions

5 Figure 4 The relationship between buserelin treatments and patient serum anti-GnRH titers. The measured absorbance at 405 nm of different plasma samples using the described ELISA procedure was transformed to the corresponding rabbit anti-GnRH titer with the aid of the standard curve. Gastroenterology  , 45-51DOI: ( /j.gastro ) Copyright © 2007 AGA Institute Terms and Conditions


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