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NCBI - Nucleotide collection (nr/nt) Affiliation Closest Match

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1 NCBI - Nucleotide collection (nr/nt) Affiliation Closest Match
Sequencing results - The cultivable moonmilk community Supplementary Table 4: The cultivable and accessible bacterial and fungal moonmilk community on solid medium Oligo is listed below. Acc.No. of cultures deposited into Genbank in current study are marked in bold. Acc.No., sequence accession number, QC, query coverage; MI, maximum identity; NCBI - Nucleotide collection (nr/nt) NCBI - 16S rRNA sequences (only Archaea and bacteria) / UNITE - ITS regions (only fungi) Affiliation Closest Match Phylum Culture (Acc.No.) Closest Match Closest Match (Acc.No.) QC (%) MI (%) Order Class Bacteria KF964472 Arthrobacter agilis KC 100 NR_ 99 Actinomycetales Actinobacteria KF964469 Arthrobacter oxydans HF Arthrobacter phenanthrenivorans NR_ KF964471 Micrococcus sp. KF Micrococcus luteus NR_ KF964459 Rhodococcus globerulus KF Nocardia globerula NR_ KF964474 Rhodococcus qingshengii KF Rhodococcus erythropolis / R. qingshengii NR_ / NR_ 100 / KF964466 Paenibacillus sp. / Exiguobacterium sp. JN / FJ 99 / Paenibacillus castaneae NR_ Bacillales Bacilli Firmicutes KF964470 FJ KF964462 Paenisporosarcina macmurdoensis JX NR_ KF964463 Paenisporosarcina sp. KF KF964458 Sporosarcina sp. KC Sporosarcina globispora / S. Psychrophila NR_ / NR_ KF964460 Sporosarcina globispora AB NR_ KF964464 Flavobacterium glaciei NR_ 98 Flavobacteriales Flavobacteria Bacteriodetes KF964461 Flavobacterium xinjiangense HQ NR_ KF964465 Flavobacterium sp. DQ KF964467 KF964475 Spingomonas sp. (faeni / mucosissima) HG / KF Sphingomonas sp. (aerolata / faeni / aurantiaca) NR_ / NR_ / NR_ 100 / 100 / Sphingomonadales Alpha-P. Proteobacteria KF964468 Pseudomonas sp. (mandelii / monteilii) KF / KF Pseudomonas mandelii NR_ Pseudomonadales Gamma-P. KF964473 JN / KF Fungi KF964484 Hypocreales HQ 92 Myrothecium roridum FJ Sordariomycetes Ascomycota KF964485 uncultured fungus KC Rhodosporidium sp. (diobovatum) / Rhodotorula mucilaginosa AB / DQ 97 / 97 Sporidiales Urediniomycetes Basidiomycota

2 Sequencing results - The natural archaeal community (cloning approach)
Supplementary Table 5: The natural archaeal moonmilk community from a typical white (MM4) and a dark moonmilk deposit (DM1), accessible via cloning and sequencing, is listed below. Acc.No. of clones deposited into Genbank in current study are marked in bold. Acc.No., sequence accession number, QC, query coverage; MI, maximum identity; S_ab score, similarity score (RDB based). NCBI - Nucleotide collection (nr/nt) Ribosomal Database Project (RDP) results Source Clone (Acc.No.) Closest Match Acc.No. QC (%) MI (%) S_ab score Genus Family Order Class Phylum MM4 KF964405 unc. Archaeon HM 99 uncultured archaeon AB294258 0.924  Thermogymnomonas  incertae sedis Thermoplasmatales  Thermoplasmata  Euryarchaeota KF964406 HM 100 0.756  KF964407 0.938  KF964408 98 0.752  KF964409 0.747  KF964410 0.925  KF964411 96 0.724  KF964412 0.735  KF964413 FJ 89 GU205479 0.961  KF964414 KC AB050205 0.946  Nitrosopumilus  Nitrosopumilaceae  Nitrosopumilales  - Thaumarchaeota KF964415 unc. Euryarchaeon EU 93 78 AM697998 0.435  KF964416 unc. bacterium JQ 87 uncultured candidate division OP11 bacterium AY193167 0.501  Microgenomates  KF964417 HM 0.730  DM1 KF964418 0.915  KF964419 AY 97 85 uncultured euryarchaeote EU731116 0.524  KF964420 0.913  KF964421 HM KF964422 0.726  KF964424 0.896  KF964425 0.751  KF964426 KC AB294260 0.962 

3 Sequencing results - The natural bacterial community (cloning approach) Supplementary Table 6: The natural bacterial moonmilk community from a typical white (MM4) and dark moonmilk deposit (DM1), accessible via cloning and sequencing, is listed below. Acc.No. of clones deposited into Genbank in current study are marked in bold. Acc.No., sequence accession number, QC, query coverage; MI, maximum identity; S_ab score, similarity score (RDB based). NCBI - Nucleotide collection (nr/nt) Ribosomal Database Project (RDP) results Source Clone (Acc.No.) Closest Match AC No. (CM) QC (%) MI (%) Acc.No. S_ab score Genus Family Order Class Phylum MM4 KF64432 Rhodococcus sp. JX 100 AB177884 0.983  Rhodococcus  Nocardiaceae  Actinomycetales  Actinobacteria  Actinobacteria KF64433 unc. bacterium JX 99 uncultured bacterium AB240251 0.819  Gp7  incertae sedis Acidobacteria KF64434 KC 98 uncultured Acidobacteria bacterium EF417701 0.876  KF64435 FN EU132542 0.896  Gaiella  Gaiellaceae  Gaiellales  KF64436 EU 95 uncultured beta proteobacterium AB252928 0.741  Betaproteobacteria  Proteobacteria KF64437 HM HM187007 0.899  Planctomycetes KF64438 FJ uncultured actinobacterium AB113594 0.789  KF64439 HM 93 DQ066982 0.618  Ignavibacterium  Ignavibacteriaceae  Ignavibacteriales  Ignavibacteria  Ignavibacteriae  KF64440 EU 92 uncultured proteobacterium EF018617 0.548  Rhizobiales  Alphaproteobacteria  KF64441 JX AY221081 0.953  DM1 KF64442 HM 97 FJ485018 0.663  Deltaproteobacteria  KF64443 HE EU715868 0.967  Gp6 KF64444 JN JN615642 0.976  KF64445 unc. Alphaproteobacterium EF unidentified soil bacteria AM168176 0.964  Pedomicrobium  Hyphomicrobiaceae  KF64446 unc. Chloroflexi bacterium JQ 96 uncultured Chloroflexi bacterium AJ582210 0.864  Chloroflexi KF64447 JX JX225068 0.823  Clostridiales  Clostridia  Firmicutes  KF64448 HM DQ906782 0.930  KF64449 JX DQ302434 0.884  KF64450 HM 94 EU617826 0.697  Rhodospirillaceae  Rhodospirillales  KF64451 GQ AF293006 0.941 

4 Sequencing results - The natural archaeal, bacterial and fungal community (DGGE-based approach) Supplementary Table 7: The natural archaeal, bacterial and fungal moonmilk community, accessible via DGGE-band excision and sequencing, is listed below. The band labels as stated in figure 6 are indicated under DGGE-band. of Acc.No. of clones deposited into Genbank in current study are marked in bold. Acc.No., sequence accession number, QC, query coverage; MI, maximum identity; S_ab score, similarity score (RDB based). NCBI - Nucleotide collection (nr/nt) Ribosomal Database Project (RDP) results Phylum DGGE-band (Acc.No.) Closest Match AC No. (CM) QC (%) MI (%) Acc.No. S_ab score Genus Family Order Class Archaea A1 / F964427 unc. Archaeon KC 99 100 uncultured crenarchaeote AB243800 0.928  Nitrosopumilus  Nitrosopumilaceae  Nitrosopumilales incertae sedis Thaumarchaeota A2 / KF964428 HM 98 uncultured archaeon AB294258 0.840  Thermogymnomonas  Thermoplasmatales Thermoplasmata Euryarchaeota A3 / KF964429 HM 0.978  A4 / KF964430 HM 0.830  A5 / KF964431 Nitrosopumilus maritimus NR_ AF050613 0.995  Bacteria B1 / KF964452 unc. bacterium JN 97 uncultured bacterium FJ535076 0.733  Methylococcaceae  Methylococcales Gammaproteobacteria Proteobacteria B2 / KF964453 0.742  B3 / KF964454 unc. betaproteobacterium KF JF266450 0.831  Thiobacter  Burkholderiales  Betaproteobacteria B4 / KF964455 Rhodococcus sp. HF Rhodococcus fascians X79187 0.987  Rhodococcus   Nocardiaceae  Actinomycetales Actinobacteria B5 / KF964456 uncultured Methylosarcina sp. JQ038199 0.718  Methylosarcina  B6 / KF964457 AJ JN850083 0.901  Nitrosospira  Nitrosomonadaceae  Nitrosomonadales Fungi F1 / KF964476 uncultured eukaryote EF uncultured Sebacina FJ792845 0.311  Sebacinaceae  Sebacinales  Agaricomycetes  Basidiomycota F2 / KF964477 Galactomyces geotrichum JQ Phaffomyces antillensis EF550262 0.395  Tetrapisispora  Saccharomycetaceae  Saccharomycetales Saccharomycetes Ascomycota F3 / KF964478 KC 95 uncultured fungus HQ755771 0.322  Gemmatimonas  Gemmatimonadaceae  Gemmatimonadales  Gemmatimonadetes  Gemmatimonadetes F4 / KF964479 Bodomorpha sp. HM 0.414  Basidiomycota  F5 / KF964480 Hypocreales sp. HQ 0.403  Hypocreales Sordariomycetes F6 / KF964481 AB uncultured Treponema sp. AB191916 0.333  Treponema  Spirochaetaceae  Spirochaetales  Spirochaetia  Spirochaetes F7 / KF964482 AB 93 0.346  F8 / KF964483 Hyphodontia rimosissima DQ 0.514 

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