1. Distinctive proliferative phase differences in gene expression in human myometrium and leiomyomata 
Hongbo Wang, MD, Mamatha Mahadevappa, PhD, Karen Yamamoto, PhD, Yan Wen, MD, Bertha Chen, MD, Janet A Warrington, PhD, Mary Lake Polan, MD, PhD, MPH 
Fertility and Sterility 
Volume 80, Issue 2, Pages (August 2003)
DOI: /S (03)

2. FIGURE 1 Flow diagram of candidate gene selection.
Wang. Gene expression in myometrium and leiomyomata.Fertil Steril 2003.
Fertility and Sterility  , DOI: ( /S (03) )

3. FIGURE 2
Hierarchical clustering of the samples and candidate genes. Each row on the y-axis represents one of the 68 candidate genes whose expression level differed significantly in a comparison of normal myometrium and myomata. The dendrogram on the y-axis clusters genes with similar expression levels. The spectrum of expression level is represented from red to green, with red representing high expression and green representing low expression. Sample clusters are shown on the x-axis. Myometrium samples have “A” codes, and matched myoma samples have “B” codes. The Pearson correlation of similarity coefficient grouped the normal myometrium and myoma into distinct groups except for the myoma of patient 7. The probe set denotes the GenBank/TIGR accession number. The P-value from Student’s t-test of each gene is indicated in the column P value. Avg FC denotes average fold change, which compares mRNA quantity in leiomyomata to mRNA quantity in matched myometrium. Positive fold changes indicate mRNA quantities that are higher in leiomyomata than myometrium; negative fold changes indicate mRNA quantities that are lower in leiomyomata than in myometrium.
Wang. Gene expression in myometrium and leiomyomata.Fertil Steril 2003.
Fertility and Sterility  , DOI: ( /S (03) )

4. FIGURE 3
RT-semiquantitative PCR analysis. One microgram of RNA from paired myoma/myometrium loaded and electrophoresed on agarose gels to examine extracellular protein S1-5 and ERα gene expression. Images of agarose gels stained with ethidium bromide showed that ERα up-regulated expression and extracellular protein S1-5 down-regulated expression in myoma tissue.
Wang. Gene expression in myometrium and leiomyomata.Fertil Steril 2003.
Fertility and Sterility  , DOI: ( /S (03) )

5. FIGURE 4
ER immunohistochemical staining. Frozen myoma and myometrium samples were subjected to ER immunohistochemical staining. ER immunohistochemical staining was performed using prediluted anti-ER monoclonal antibody. ER immunohistochemical staining is reported as the percentage of nuclei staining positive as well as the intensity of the staining. The intensity of staining multiplied by the percentage of nuclei stained for each sample yielded a score representing ER protein expression. Brown represents positive staining (arrow) in a representative sample. (A), myometrium; (B), leiomyoma.
Wang. Gene expression in myometrium and leiomyomata.Fertil Steril 2003.
Fertility and Sterility  , DOI: ( /S (03) )