1. Microscope Use

2. Scale

3. Simple Microscope Light passes through only 1 lens.
Example: magnifying glass

4. Compound Microscope
Lets light pass through an object and then through two or more lenses.

5. Parts of the Microscope
Drag the box to view the correct answer underneath.
Ocular lens (Eyepiece)
Parts of the Microscope
Body Tube
Nosepiece
Arm
Low Power Objective
High Power Objective
Stage
Stage Clips
Coarse Adjustment
Diaphragm
Fine Adjustment
Light
Always carry a microscope with one hand holding the arm and one hand under the base.
Base

6. Microscope Parts & Their Functions
Drag the box to view the correct answer underneath.
Controls the amount of light entering the microscope
diaphragm
Makes large changes in focus
Coarse adjustment
Makes small changes in focus
Fine adjustment
Holds the slide in position
Stage clips
Has the greatest magnification
High power objective
Has the least magnification
Low power objective

7. Fill in the table on your worksheet.
What’s my power?
To calculate the power of magnification, multiply the power of the ocular lens (eyepiece) by the power of the objective lens.
What are the powers of magnification for each of the objectives we have on our microscopes?
Fill in the table on your worksheet.

8. Microscope Care Always carry with 2 hands (arm and base)
Only use lens paper for cleaning
Do not force knobs
Always store covered
Keep objects clear of desk and cords

9. Using the Microscope Lower stage all the way
Rotate nosepiece and click the low-power objective lens(shortest) into position over the stage
Place the slide on the microscope
Look into the eyepiece
Use the Coarse Adjustment knob to locate the object
Use the Fine Adjustment to sharpen the image

10. How to make a wet-mount slide …
1 – Get a clean slide and coverslip from your teacher.
2 - Place the object you are viewing in the center of the slide
3 – Place ONE drop of water on the object. Don’t use too much or the water will run off the edge and make a mess!
4 – Place the edge of the cover slip on one side of the water drop.
5 - Slowly lower the cover slip on top of the drop.
Cover Slip
Lower slowly
6 – Tap out the air bubbles.
7- Use a paper towel to blot up any excess water.

11. Stereoscopic Microscope
Gives a three dimensional view of an object. (Examples: insects and leaves)
Used for dissections

12. Electron microscopes – use a beam of electrons instead of a beam of light to magnify the image

13. Electron Microscopes
can achieve 3D images using electrons

14. The Scanning Electron Microscope
produces a 3-dimensional image of specimen’s surface features
spider
head of a butterfly

15. Scanning electron microscopy (SEM)
Types of specimens:
-Whole organisms
-Natural tissue surfaces
-Exposed tissue structure
A flea magnified X
What is this?

16. Transmission electron microscopy (TEM).
Allows the observation of molecules within cells
Allows the magnification of objects in the order of 100, 000’s.

17. Transmission electron microscope (TEM)
Provides for detailed study of the internal ultrastructure of cells
a beam of electrons
is transmitted through
the specimen for
a 2D view
Longitudinal
section of
cilium
Cross section
of cilium
1 µm
Figure 6.4 (b) cilia on rabbit lungs

18. Transmission electron microscope
Chloroplast from a tobacco leaf
H1N1 virus