1. Surveillance of Foodborne Enteropathogen in Brazil
National Reference Laboratory
Laboratory of Enterobacteria
Oswaldo Cruz Institut-FIOCRUZ
Dalia dos Prazeres Rodrigues, Ph.D

2. Ministry of Health Secretary of Health Surveillance
National Reference Laboratory
Regional Reference Laboratories
State Reference Laboratories (PHL)
Municipal Labs
Border Labs
Local Labs
National Epidemiological Surveillance Network
National Environmental Surveillance Network
National Sanitary Surveillance Network
National Health Assistance Network
Collaborating Centre
National Division

3. Regional laboratory

4. Public Health Laboratories
Ministry of Health- Laboratories Coordination
National Reference
Laboratory
IOC / FIOCRUZ
Exotic serovars
Multidrug resistant
strains
Epidemic strains
Surveillance of emergent species/serovars
Public Health Laboratories

5. National Reference Laboratory-activities
Sampling:
Human source and foodborne outbreaks by PHL;
S.Typhi, V.cholerae O1 and non O1, Aeromonas sp. and, V.parahaemolyticus strains;
All Salmonella spp. isolates from animal, feed and foodstuff products by Ministry of Agriculture Laboratories;
Serotyping activities-Salmonella spp:
Isolates from Public and Private Universities;
Isolates from private Institutions from Food, Animal, Feed, Environment, and Foodstuff ;
Activities carried out by NRL
Serotyping; Salmonella spp., Shigella sp. , E.coli, V.parahamolyticus, Listeria monocytogenes,
Surveillance of antimicrobial susceptibility and molecular characterization;
Phage typing: S.Typhi, S.Typhimurium, S.Enteritidis, S.Hadar;
PCR- virulence genes
Sub typing - PFGE

6. Total strains isolated from human source received from PHL
2001
2002
2003
2004
2005
2006
8,161
9,826
10,679
13,770
17,495
17,020

7. Activities carried out by NRL-2006-2007 PFGE
Shigella flexneri: Total analyzed strains: 50

8. Pulsenet activities Salmonella Enteritidis Salmonella Typhimurium
Salmonella 1,4,12:i:-

9. V.parahaemolyticus Enzyme: Not I Total analyzed strains: 35 PE 2001 12
State
Ano
Strains
No.
Serovars
PCR
Kanagawa
Tdh PE
2001 12
O3:KUN + AL
2002 8
O3:K6 CE 18
O3 K6

10. V.parahaemolyticus

11. Activities carried out by NRL- PFGE: 2006-2007
Outbreak
Surveillance
S.Panama (>185 human cases)
State: Pernambuco (total: 8 strains)
S.Enteritidis (32 human cases)
State : Bahia (total: 14 strains)
S. Enteritidis (? Human cases)
State: Roraima (total: 13 strains)
S.Typhimurium DT120 (> 50 human cases)
State: Rio de Janeiro (total: 5 strains)
S.Enteritidis (human, food, animal)
Total analyzed strains: 35
S.Heidelberg (food)
Total analyzed strains: 15
S.Typhimurium DT 104, DT120, DT193 (human, food, animal, feed)
Total analyzed strains: 40
S. Agona (human, food, feed, animal)
Total analyzed strains: 60
Salmonella spp. strains isolated from sewage waters in
Pan American Ville

12. S.Panama
S.Agona

13. Estimated activities: 2007-2008
GSS-Level II course- strengthen the PHL into classical and PCR diagnostic of Campylobacter sp. and antigenic diagnostic of Salmonella spp. and other enteropathogens
PFGE course to Regional Laboratories;
Implemented activities between National Reference Laboratory/IOC and Biomanguinhos/FIOCRUZ providing polyvalent and monovalent antisera;
Standardize PFGE to Aeromonas hydrophila;
Standardize PFGE to Listeria monocytogenes
Attend the collaborative Pulsenet activities;