1. Quantitative analysis of follistatin (FST) promoter methylation in peripheral blood of patients with polycystic ovary syndrome 
Qing Sang, Shaozhen Zhang, Shien Zou, Huan Wang, Ruizhi Feng, Qiaoli Li, Li Jin, Lin He, Qinghe Xing, Lei Wang 
Reproductive BioMedicine Online 
Volume 26, Issue 2, Pages (February 2013)
DOI: /j.rbmo
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2. Figure 1
A schematic diagram of CpG sites in the FST promoter and 5′-UTR region. CpG sites are depicted by lollipop markers. Primer-binding sites targeted in this study are shown below the diagram. → Represents forward primers and ← represents reverse primers. The number of lollipop markers is not indicative of detected CpG sites: 140 CpG sites were identified in the FST promoter and 5′-UTR region.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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3. Figure 2
Comparison of methylation at each CpG site in the FST promoter and 5′-UTR region of peripheral blood from 40 PCOS patients and 40 controls. (A, B, C, D) Four different amplicons in the promoter and 5′-UTR regions of the FST gene. Values are mean±SE. ∗P<0.001.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2012 Reproductive Healthcare Ltd. Terms and Conditions

4. Figure 3
Comparison of methylation at CpG 22,23 in amplicon 1 of the FST gene between 90 PCOS patients and 80 controls. Black horizontal lines represent the mean methylation percentage.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2012 Reproductive Healthcare Ltd. Terms and Conditions

5. Figure 4
Comparison of methylation of CpG units in amplicon 1 of the FST gene in endometrial tissues sampled from 24 PCOS patients and 24 controls. Values are mean±SE.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2012 Reproductive Healthcare Ltd. Terms and Conditions

6. Figure 5
Relative mRNA expression of the FST gene in endometrial tissue sampled from PCOS patients and controls. The final data were double normalized using human actin and GAPDH as controls.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2012 Reproductive Healthcare Ltd. Terms and Conditions