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Volume 6, Issue 3, Pages (May 2013)

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1 Volume 6, Issue 3, Pages 716-728 (May 2013)
Genetic and Physiological Analysis of a Novel Type of Interspecific Hybrid Weakness in Rice  Chen Chen, Hao Chen, Jun-Xiang Shan, Mei-Zhen Zhu, Min Shi, Ji-Ping Gao, Hong-Xuan Lin  Molecular Plant  Volume 6, Issue 3, Pages (May 2013) DOI: /mp/sss146 Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

2 Figure 1 Phenotypic Characterization of Hybrid Weakness in Rice Plants. (A, B) The morphology of NIL(hwi1) and NIL(Hwi1) at the seedling stage (A) and the heading stage (B). CPhenotypic evaluations of yield-related traits of NIL(hwi1) and NIL(Hwi1). Twenty independent individuals were evaluated for quantification. ** P ≤ 0.01; ns, no significance. Scale bar = 10cm in (A) and (B). Molecular Plant 2013 6, DOI: ( /mp/sss146) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

3 Figure 2 Root Developmental Defects in Plants Expressing Hybrid Weakness. ATime-course analysis of root number in NIL(hwi1) and NIL(Hwi1). (B, C) Cross-sections of basal nodes, the tissue from which crown roots initiate, of NIL(hwi1) (B) and NIL(Hwi1) (C). (D, E) Mature embryo structure of NIL(hwi1) (D) and NIL(Hwi1) (E). Scale bar = 200 μm; yellow arrows indicate the crown root primordia. Molecular Plant 2013 6, DOI: ( /mp/sss146) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

4 Figure 3 Responses to Various Plant Hormone Treatments of NIL(hwi1) and NIL(Hwi1). ARoot numbers of NIL(hwi1) and NIL(Hwi1) seedlings incubated in liquid nutrient solution with or without plant hormones for 30 d. BPlant height of NIL(hwi1) and NIL(Hwi1) seedlings incubated in liquid nutrient solution with or without plant hormones for 30 d. Ten individuals were evaluated for phenotypic quantification. Molecular Plant 2013 6, DOI: ( /mp/sss146) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

5 Figure 4 RT–PCR Assays to Detect the Expression of Root Development-Related Genes in NIL(hwi1) and NIL(Hwi1). ‘R’ indicates the roots, ‘N’ indicates the basal nodes. Molecular Plant 2013 6, DOI: ( /mp/sss146) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

6 Figure 5 Fine Mapping of the Hwi1 Gene (A–D) and the Expression of Candidate Genes (E, F). ALinkage map of the wild rice chromosome substitution region (shown by the solid black bar) on chromosome 11. The Hwi1 gene was delimited between flanking markers RM26153 and RM Numbers shown between adjacent markers indicate the genetic distance in cM. (B, C) Fine mapping of the Hwi1 gene. Numbers under each marker indicate the number of recombinants between the marker and the Hwi1 gene. Scale bars of the physical maps are 10 kb in (B) and 2 kb in (C). The Hwi1 gene was localized to the region defined by CAPS56 and M4 and two candidate genes, LOC_Os11g07230 and LOC_Os11g07240, within this region are shown in (D). The expression level of LOC_Os11g07230 (E) and LOC_Os11g07240 (F) in the basal nodes of NIL(hwi1) and NIL(Hwi1) as determined by real-time PCR. Molecular Plant 2013 6, DOI: ( /mp/sss146) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

7 Figure 6 Phylogenetic Tree of LOC_Os11g07240 Homologs Found in Plants.
The prefix of each protein indicates the species name. Species names are abbreviated as follows: Oryza sativa ssp. japonica (Os), O. sativa ssp. indica (OsI), O. glaberrima (Og), Hordeum vulgare (Hv), Zea mays (Zm), Triticum aestivum (Ta), Sorghum bicolor (Sb), Brachypodium distachyon (Bd), Arabidopsis thaliana (At), Glycine max (Gm), Medicago truncatula (Mt), Populus trichocarpa (Pt), Ricinus communis (Rm), Vitis vinifera (Vv). Molecular Plant 2013 6, DOI: ( /mp/sss146) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

8 Figure 7 The Expression Level of Pathogen-Related Genes (PRs) and Phytoalexins Biosynthesis-Related Genes in the Basal Nodes of NIL(hwi1) and NIL(Hwi1). Molecular Plant 2013 6, DOI: ( /mp/sss146) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions


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