1. Rat bone marrow mesenchymal stem cells improve regeneration of thin endometrium in rat 
Zhao Jing, M.D., Zhang Qiong, M.D., Wang Yonggang, M.D., Li Yanping, M.D. 
Fertility and Sterility 
Volume 101, Issue 2, Pages e3 (February 2014)
DOI: /j.fertnstert
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2. Figure 1
(A) Blank group; (B) experimental group; (C) control group. The morphology observation of the endometrium with hematoxylin and eosin (H & E) staining of the three groups (H & E, ×200 μm). Significantly thicker endometrial lining was observed in the experimental group compared with that of the control group (P<.01), and there was no significant difference between experimental group and the normal group.
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3. Figure 2
Expression of vimentin (A), cytokeratin (B), integrin-β3 (C), leukemia inhibitor factor (LIF) (D), and bromodeoxyuridine (BrdU) (E) with immunohistochemistry. The expression of cytokeratin, vimentin, integrin-β3, and LIF in experimental group were significantly stronger than that of control groups (P<.05), and there was no significant difference between the experimental group and the blank group (P>.05). There were BrdU-positive cells only in the experimental group, with significant difference compared with the blank group and the control group (P<.05).
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4. Figure 3
Expression of cytokeratin, vimentin, integrin αγβ3, and leukemia inhibitor factor (LIF) with Western blot. Blank group = 1; experimental group = 2; control group = 3. (A, B) The result of Western blot. (C) The relative protein levels, expressed by the gray value ratio (% adjusted volume). Results showed that the expression of cytokeratin, vimentin, integrin αγβ3, and LIF of the experimental group was significantly stronger compared with the control group (P<.05), and there was no significant difference between the experimental group and the blank group (P>.05).
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5. Figure 4
(A–D) Expression of fibroblast growth factor-β (bFGF) messenger RNA (mRNA), interleukin (IL)-6 mRNA, IL-1β mRNA, and tumor necrosis factor-α (TNF-α) mRNA with real time-polymerase chain reaction (PCR). (E) The relative mRNA content, expressed by the gray value ratio (% adjusted volume). Blank group = 1, 1’; experimental group = 2, 2’; control group = 3, 3’; 1’–3’ = internal reference glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Results showed that the expression of bFGF mRNA and IL-6 mRNA was significantly up-regulated in the experimental group compared with the control group (P<.05), and the expression of IL-1β mRNA and TNF-α mRNA was significantly down-regulated in the experimental group compared with the control group (P<.05).
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6. Supplemental Figure 1
Differentiation and flow cytometry analysis of rat bone marrow mesenchymal stem cells. (A1, A2) Osteoblasts differentiation (alizarin red S staining); (A3, A4) Adipocytes differentiation (oil red O staining). A1 and A3 were the experimental group, using conditioned medium; A2 and A4 were the control group, using basic medium. (B1–B4) The phenotype of rat bone marrow mesenchymal stem cells was shown to be positive for CD90 and CD73 and negative for CD34 and CD45.
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