1. by Dörte Bechtel, Julia Kurth, Claus Unkel, and Ralf Küppers
Transformation of BCR-deficient germinal-center B cells by EBV supports a major role of the virus in the pathogenesis of Hodgkin and posttransplantation lymphomas
by Dörte Bechtel, Julia Kurth, Claus Unkel, and Ralf Küppers
Blood
Volume 106(13):
December 15, 2005
©2005 by American Society of Hematology

2. LMP2A expression in CB-LCLs.
LMP2A expression in CB-LCLs. (A) LMP2A protein expression was determined by Western blot analysis. The peripheral blood B-cell–derived LCL and LCL were used as positive controls. The protein lysate of the EBV-negative cell line BL41 was blotted as a negative control. All LCLs analyzed were consistently positive for LMP2A. (B) β-actin was used as a loading control.
Dörte Bechtel et al. Blood 2005;106:
©2005 by American Society of Hematology

3. Flow cytometric analysis of Ig light chain surface expression with fluorescent liposomes.
Flow cytometric analysis of Ig light chain surface expression with fluorescent liposomes. Cells were stained with anti-Igκ– and anti-Igλ–streptavidin antibodies and then incubated with biotin-coupled, Cy5-filled liposomes. (A-D) Shown are examples of a sIgλ+ cell line (A; CB-LCL 6-1), a sIgκ+ cell line (B; CB-LCL 5-B40), and a sIg-negative cell line (C-D; CB-LCL 5-7-1).
Dörte Bechtel et al. Blood 2005;106:
©2005 by American Society of Hematology

4. VHDHJH and DHJH gene rearrangements of the 2 Ig-crippled cell lines.
VHDHJH and DHJH gene rearrangements of the 2 Ig-crippled cell lines. (A,C) Shown are the VHDHJH gene sequences of CB-LCL 4-17 and CB-LCL from the 5′ end of FR3 to the start of the JH primer sequence. (B,D) The DHJH gene sequences are depicted from upstream of the recombination signal sequences (RSS; composed of nonamer, 12 base pair [bp] spacer and heptamer) 5′ of the DH segments to the start of the JH primer. (A,B) CB-LCL 4-17, (C,D) CB-LCL N represents N nucleotides. (A) The 2 point mutations giving rise to a nonsense codon in CB-LCL 4-17 are located within the first codon of FR3 and are underlined. (C) CB-LCL contains a 3-bp and a 51-bp deletion in FR3 that are indicated by black boxes. The 51-bp deletion renders FR3 of CB-LCL nonfunctional. In this sequence a DH segment could not be identified but may be part of the sequence marked by N.
Dörte Bechtel et al. Blood 2005;106:
©2005 by American Society of Hematology