1. CRISPR-Cas9 gene drive system

2. What is a gene drive?
Elements that increase the odd that they will be inheritance
Mendelian inheritance
Gene drive inheritance
Natural gene drives
Synthetic gene drives
-Transposable elements
-Maternal effect killers
-Homing endonucleases
Limitations: Engineering nucleases to cut new targets

3. CRISPR/CAS9 system Jennifer Doudna Guide RNA Spacer PAM sequence
Protospacer
Cas9
Genomic DNA

4. CRISPR/CAS9 gene drive system
Kevin Esvelt A D B E C F

5. Inheritance
Esvelt et al. 2014

6. Limitations 1. Asexual organisms can`t be affected
2. Many generations to spread through populations Ineffective in long lived organisms
3. Drive-mediated genome alterations aren`t permanent

7. Capabilities 1. Reversibility 2. Population suppression
Esvelt et al. 2014
2. Population suppression
Deleterious changes
Bias the population towards one sex
3. Complex alterations
Sensitize
Speciate

8. Applications Prevent mosquitoes from transmitting malaria AgCPA
Valentino Gantz
AgCPA

9. THANKS FOR YOUR ATTENTION!