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Robert J. Pitsch, MD, David J. Minion, MD, Margaret L

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1 Platelet-derived growth factor production by cells from Dacron grafts implanted in a canine model 
Robert J. Pitsch, MD, David J. Minion, MD, Margaret L. Goman, BS, John A. van Aalst, MD, Paul L. Fox, PhD, Linda M. Graham, MD  Journal of Vascular Surgery  Volume 26, Issue 1, Pages (July 1997) DOI: /S (97) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 Southern blots of SMC and EC PDGF A-chain and GAPDH mRNA amplified by RT-PCR. Aortic and graft ECs and SMCs are from a representative dog. Images are computer-enhanced. A-chain/GAPDH numbers represent the ratios of signal intensities before enhancement. Journal of Vascular Surgery  , 70-78DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 2 Graph of RT-PCR results shows PDGF A-chain/GAPDH signal intensity. Graft ECs express lower levels of PDGF A-chain mRNA than aortic ECs, whereas graft SMCs consistently express higher levels than aortic SMCs. Journal of Vascular Surgery  , 70-78DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 3 Immunohistochemical analysis using antibody to PDGF A-chain (original magnification, ×400). Aortic section (A) has less staining of SMCs than graft section (B). Journal of Vascular Surgery  , 70-78DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5 Fig. 3 Immunohistochemical analysis using antibody to PDGF A-chain (original magnification, ×400). Aortic section (A) has less staining of SMCs than graft section (B). Journal of Vascular Surgery  , 70-78DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

6 Fig. 4 Immunohistochemical analysis using antibody to PDGF B-chain (original magnification, ×400). Aortic section (A) has less staining of SMCs than graft section (B). Journal of Vascular Surgery  , 70-78DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

7 Fig. 4 Immunohistochemical analysis using antibody to PDGF B-chain (original magnification, ×400). Aortic section (A) has less staining of SMCs than graft section (B). Journal of Vascular Surgery  , 70-78DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions


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