1. Molecular Genetics Are we controlled by our DNA?
Refer to pg in Holtzclaw, Ch in Campbell

2. Last year...
What do you remember of DNA Replication and Protein Synthesis?
DNA > mRNA > protein
genetic info message action molecule
mRNA processing
DNA Replication
Transcription
Translation

3. So What? Making the big connections...
How many cells do you have?
Compare the DNA in each of your cells
Compare the protein products in each of your cells...
How does your body KNOW when to make some proteins and not others?
THIS is gene expression
EVERY single one of your cells have the same genetic code... Must be able to turn some genes on and some genes off...

4. So What?
Model Organism: The Fruit Fly
3 min video clip

5. So What? More flies! - Using Gene expression to study eye development
3 minute video clip

6. What do you think? Are you controlled by your DNA?
What are the implications of studying Gene expression?
1) Free Write (3 min)
2) Learning Walk (5 min)
3) No Hands Discussion (5 min)

7. Review last year…
Learning Intentions!

8. Gene Expression You must know:
The functions of the three parts of an operon
The role of repressor genes in operons
The impact of DNA methylation and histone acetylation on gene expression
The role of gene regulation in embryonic development and cancer.

9. Gene Expression Rather important, don’t you think?:
The functions of the three parts of an operon
The role of repressor genes in operons
The impact of DNA methylation and histone acetylation on gene expression
The role of gene regulation in embryonic development and cancer.

10. The Operon – p. 131 in Holtz. What is it? Unit of genetic function
Found in bacteria/phages/some eukaryotes
Consists of:
Promotor – site of RNA polymerase binding
Operator – controls access of RNA polymerase within the promotor
Genes of the Operon –regulated together by the operon

11. Let’s Make the Connections!
Regulatory Genes
What do they do?
Produce repressor proteins that bind to the operator of an operon to block RNA polymerase from transcribing
Repressible Operon
Usually “on,” can be inhibited...
Inducible Operon
Usually “off,” can be induced...
Let’s Make the Connections!

12. The Try Operon – Repressible

13. The Lac Operon – Inducible

14. Let’s make sure we get this…
Handout…
Act Out!