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Human cells stained with an antibody for the Tubulin protein

Published byΘεοδοσία Δημαράς Modified over 5 years ago

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Presentation on theme: "Human cells stained with an antibody for the Tubulin protein"— Presentation transcript:

1 Human cells stained with an antibody for the Tubulin protein
Image from Molecular Probes

2 GFP-RAS2 in living Saccharomyces cerevisiae

3 Images from Roger Tsein’s Lab

4

5 Making a Fusion Protein:
Yak1 Gene: DNA: AAAGATGAATCAG ... ACATCGTGACCTA... Protein: N-Met-Asn-Gln-…-Thr-Ser-C GFP Gene: DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC... Protein: N-Met-Ser-Lys-… -Leu-Lys-C Yak1 GFP

6 Making a Fusion Protein:
Yak1 Gene: DNA: AAAGATGAATCAG ... ACATCGTGACCTA... GFP Gene: DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC... Fusion Gene: AAAGATGAATCAG ... ACATCGATGAGTAAA ... CTGAAATAATGACCTA...

7 Making a Fusion Protein:
Yak1 Gene: DNA: AAAGATGAATCAG ... ACATCGTGACCTA... Protein: N-Met-Asn-Gln-…-Thr-Ser-C GFP Gene: DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC... Protein: N-Met-Ser-Lys-… -Leu-Lys-C Yak1 GFP N C Fusion Gene: AAAGATGAATCAG ... ACATCGATGAGTAAA ... CTGAAATAATGACCTA... Fusion Protein: N-Met-Asn-Gln-…-Thr-Ser-Met-Ser-Lys-… -Leu-Lys-C

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