1. Circulating Exosomal miR-17-5p and miR-92a-3p Predict Pathologic Stage and Grade of Colorectal Cancer 
Fangfang Fu, Weiqin Jiang, Linfu Zhou, Zhi Chen 
Translational Oncology 
Volume 11, Issue 2, Pages (April 2018)
DOI: /j.tranon
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2. Figure 1
Spleen injection assay to compare tumorigenicity and metastatic potential. (A) Tumorigenicity and metastatic potential of NCM460, SW480, and SW620 cells. Each group (n = 8) of mice was injected IS with 5E+06 cells. After euthanasia, autopsies were performed 4 weeks after injection. (B) Metastases analyzed. (C) Mean weights of liver following IS injection are shown.
Translational Oncology  , DOI: ( /j.tranon )
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3. Figure 2
Characteristics of exosomes from cell lines. (A) Representative TEM photograph of exosomes (scale bar, 200nm). (B) Exosome size and particle number were analyzed with TRPS. (C) Western blot of exosomes and cells for CD63 and β-actin.
Translational Oncology  , DOI: ( /j.tranon )
Copyright © 2018 The Authors Terms and Conditions

4. Figure 3
MiRNA expressions profiles of exosomes. (A) Profiling of small RNAs in exosome samples. (B) A Venn diagram showing the co-expressed and specifically expressed miRNAs in exosomes. (C) Heat map of sequencing results. Gene expression data obtained using next-generation sequencing on the Illumina HiSeq 2500 platform. P < .05 and fold change > 2 were considered significant
Translational Oncology  , DOI: ( /j.tranon )
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5. Figure 4
GO annotation and KEGG pathway analysis. (A) The top GO terms of predicted targets belong to the dysregulated expressed miRNAs. (B) Pathway analysis of differential expression genes by KEGG.
Translational Oncology  , DOI: ( /j.tranon )
Copyright © 2018 The Authors Terms and Conditions

6. Figure 5
Validation of miRNA expression in cells and exosomes. The expression levels of 11 candidate differentially expressed miRNAs in cells (A) and exosomes (B) confirmed by qRT-PCR (two-tailed Student's t test) (error bars represent means ± SEM). Experiments were performed in triplicate. P< .01.
Translational Oncology  , DOI: ( /j.tranon )
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7. Figure 6
Circulating exosomal miR-17-5p and miR-92a-3p levels correlated with pathologic stage and grade in CRC patients. (A) The size distribution and particle concentration of purified exosomes in different groups (two-tailed Student's t test) (error bars in graphical data represent means ± SEM). (B) ROC curves to differentiate CRC patients from NCs. (C) ROC curves to differentiate metastasis from nonmetastasis. (D and E) Circulating exosomal miR-17-5p and miR-92a-3p levels in clinical CRC serum specimens tested by qPCR (Mann-Whitney U test; error bars in graphical data represent means ± SEM; P < .05). (F and G) Significant correlation of exo-miR level with CRC pathologic stage (P value < .0001). A Spearman Pearson correlation was used to evaluate performance.
Translational Oncology  , DOI: ( /j.tranon )
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8. Figure 7
Exosomal miR-17-5p and miR-92a-3p levels in several other colon cancer cell lines with different gene mutations and different ability of metastasis. (A) Gene mutations and metastasis ability of CRC cell lines. (B) Exosomal miR-17-5p levels in CRC cell lines classified by different gene mutations (error bars in graphical data represent means ± SEM). (C) Exosomal miR-17-5p levels in CRC cell lines classified by invasive ability and metastasis potentials (one-way ANOVA) (error bars in graphical data represent means ± SEM). (D) Exosomal miR-92a-3p levels in CRC cell lines classified by different gene mutations (error bars in graphical data represent means ± SEM). (E) Exosomal miR-92a-3p levels in CRC cell lines classified by invasive ability and metastasis potentials (one-way ANOVA) (error bars in graphical data represent means ± SEM).
Translational Oncology  , DOI: ( /j.tranon )
Copyright © 2018 The Authors Terms and Conditions