1. Proteome analysis of stratum corneum from atopic dermatitis patients by hybrid quadrupole-orbitrap mass spectrometer 
Jun-Ichi Sakabe, PhD, Koji Kamiya, MD, PhD, Hayato Yamaguchi, MD, Shigeki Ikeya, MD, Takahiro Suzuki, MD, Masahiro Aoshima, MD, Kazuki Tatsuno, MD, Toshiharu Fujiyama, MD, Masako Suzuki, Tsuyoshi Yatagai, MD, Taisuke Ito, MD, PhD, Toshiyuki Ojima, MD, PhD, Yoshiki Tokura, MD, PhD 
Journal of Allergy and Clinical Immunology 
Volume 134, Issue 4, Pages e8 (October 2014)
DOI: /j.jaci
Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2. Fig 1
Characterization of patients with AD or IV by liquid chromatography (LC)/MS/MS analysis and immunohistochemistry. A, Representative LC/MS/MS analysis of SC. MS spectra were examined in the individual subjects. Note that nonspecific signals, such as polymer and contaminants, were not detected. Substances were detected by using the Mascot search engine (Matrix Science, London, UK; version 2.4) against SwissProt database of human. The amounts of individual proteins were semi-quantified by using the Proteome Discoverer v.1.4 software (Thermo Fisher Scientific). B, Comparison of FLG amounts between normal healthy subjects and patients with EAD, IAD, and IV. FLG quantity was decreased in patients with EAD, IAD, and IV than in normal subjects (**P < .01). C, Representative hematoxylin and eosin staining histopathology of the epidermis and dermal eccrine sweat glands in a normal subject and a patient with EAD. Scale bar is 100 μm (top). Representative mmunohistochemical staining for GCDFP15 (bottom). Red arrowheads, acrosyringium. Scale bar is 100 μm.
Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci )
Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions