1. Butler Chapter 13 DC Applicants Danielle Aguirre Toni Leggett
Shavan Young
Danielle Hoffman
Travonna Carter
Calvin Lee

2. DNA Detection Methods
Sliver staining and fluorescence techniques to detect STRs
Present – Fluorescence based detection

3. Fluorophores

4. Fluorophores

5. Fluorescence Detection

6. with color contributions Normalized Fluorescent Intensity
520
540
560
580
600
620
640
WAVELENGTH (nm)
100 80 60 40 20
310 Filter Set F
with color contributions
5-FAM
JOE
NED
ROX
Laser excitation
(488, nm)
Normalized Fluorescent Intensity
Figure 13.4, J.M. Butler (2005) Forensic DNA Typing, 2nd Edition © 2005 Elsevier Academic Press

8. ABI Prism 310 Genetic Analyzer

9. ABI 3100 multi-capillary system

10. FMBIO II gel scanner

11. Questions?