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Published byMillicent Booth Modified over 6 years ago
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Butler Chapter 13 DC Applicants Danielle Aguirre Toni Leggett
Shavan Young Danielle Hoffman Travonna Carter Calvin Lee
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DNA Detection Methods Sliver staining and fluorescence techniques to detect STRs Present – Fluorescence based detection
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Fluorophores
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Fluorophores
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Fluorescence Detection
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with color contributions Normalized Fluorescent Intensity
520 540 560 580 600 620 640 WAVELENGTH (nm) 100 80 60 40 20 310 Filter Set F with color contributions 5-FAM JOE NED ROX Laser excitation (488, nm) Normalized Fluorescent Intensity Figure 13.4, J.M. Butler (2005) Forensic DNA Typing, 2nd Edition © 2005 Elsevier Academic Press
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ABI Prism 310 Genetic Analyzer
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ABI 3100 multi-capillary system
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FMBIO II gel scanner
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Questions?
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