Presentation is loading. Please wait.

Presentation is loading. Please wait.

Platelets play important roles in the late phase of the immediate hypersensitivity reaction  Risa Tamagawa-Mineoka, MD, PhD, Norito Katoh, MD, PhD, Saburo.

Published byJean-Bernard Normandin Modified over 6 years ago

Similar presentations

Presentation on theme: "Platelets play important roles in the late phase of the immediate hypersensitivity reaction  Risa Tamagawa-Mineoka, MD, PhD, Norito Katoh, MD, PhD, Saburo."— Presentation transcript:

1 Platelets play important roles in the late phase of the immediate hypersensitivity reaction 
Risa Tamagawa-Mineoka, MD, PhD, Norito Katoh, MD, PhD, Saburo Kishimoto, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 123, Issue 3, Pages e9 (March 2009) DOI: /j.jaci Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Changes in ear swelling responses after ovalbumin (OVA) challenge. Ear thickness was measured in the ears of mice (6 per group) after administration of APAS or CS (A), or busulfan or vehicle (B). The ear weight was examined 1 hour (C) and 24 hours (D) after challenge. ∗∗P < .01 vs CS or vehicle administration in OVA-challenged mice. NS, Not significant. Journal of Allergy and Clinical Immunology  , e9DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Histologic examination of ear skin. Skin tissue was excised 1 hour (A-D) and 24 hours (E-H) after ovalbumin challenge. A and E, Injection of vehicle alone with CS administration. B and F, Injection of ovalbumin with CS administration. C and G, Injection of vehicle alone with APAS administration. D and H, Injection of ovalbumin with APAS administration. Hematoxylin and eosin. Original magnification ×200. Bar = 50 μm. Journal of Allergy and Clinical Immunology  , e9DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Effect of platelet restoration on ear swelling responses and leukocyte infiltration. Mice (6 per group) were injected intravenously with platelet suspension or vehicle. Ear swelling responses (A) and the number of infiltrated cells (B) were examined. Data are shown as percentages of the basal number of cells in the dermis 24 hours after challenge ∗∗P < .01 vs mice injected with vehicle. OVA, Ovalbumin. Journal of Allergy and Clinical Immunology  , e9DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Necessity of platelet P-selectin for cutaneous leukocyte recruitment. P-selectin expression on platelets (A) and the number of platelet-leukocyte complexes (B) were examined. Platelets isolated from P-selectin knockout (P-selectin–/–) mice (C) or treated with anti–P-selectin antibody (D) were injected. Data are shown as percentages of basal values in sham-sensitized mice or in mice injected with vehicle (5 mice per group). ∗P < .05; ∗∗P < .01. OVA, Ovalbumin; WT, wild-type. Journal of Allergy and Clinical Immunology  , e9DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 Effect of platelet-derived soluble factors on leukocyte recruitment into skin. Washed platelets were stimulated with TRAP. The activated platelet supernatant was injected into ear skin of APAS-treated mice (5 per group) just before challenge, after adding anti–MIP-1α, anti-RANTES, anti-TARC, or isotype control antibody to the supernatant. The supernatant of platelets fixed without stimulation with TRAP served as a control. ∗∗P < .01. Journal of Allergy and Clinical Immunology  , e9DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig 6 Effect of antiplatelet drugs on ear swelling responses and leukocyte infiltration. The ear weight was examined 1 hour (A) and 24 hours (B) after challenge, and skin tissue was examined 24 hours after challenge (C; 5 mice per group). Data are shown as the percentage of the basal number of cells in the dermis after vehicle administration. ∗∗P < .01. NS, Not significant. Journal of Allergy and Clinical Immunology  , e9DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Changes in ear swelling responses after ovalbumin (OVA) challenge
Changes in ear swelling responses after ovalbumin (OVA) challenge. The ear weight of W/Wv mice (A) and that of mice injected with APAS (C) or challenged (E) repeatedly and the ear thickness of mice injected with neuraminidase (B) were examined. The myeloperoxidase (MPO) and eosinophil peroxidase activities of the skin tissue were measured (D). Six mice per group. ∗∗P < .01 vs vehicle administration in OVA-challenged mice. Journal of Allergy and Clinical Immunology  , e9DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

9 Effect of platelet addition on ear swelling responses and chemokine release from in vitro ovalbumin-challenged platelets. The ear weight of mice without platelet depletion (6 per group) was examined after injection of platelet suspension (A). The levels of MIP-1α, RANTES, and TARC were measured in triplicate in the supernatant of platelets treated with serum of sensitized mice and ovalbumin (B). ∗P < .05. NS, Not significant. Journal of Allergy and Clinical Immunology  , e9DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

10 Effect of platelet restoration on formation of platelet-leukocyte complexes. Mice (5 per group) were injected with platelet suspension obtained from ovalbumin (OVA)-sensitized or sham-sensitized mice, or P-selectin–deficient mice. The number of platelet-leukocyte complexes in blood was examined. Data are shown as percentages of basal values in sham-sensitized mice (A). Chemokine levels were measured in triplicate in the supernatant of P-selectin–deficient platelets (B). ∗P < .05; ∗∗P < .01. WT, Wild-type. NS, Not significant. Journal of Allergy and Clinical Immunology  , e9DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

11 Effect of platelet-derived soluble factors on leukocyte recruitment into skin. The activated platelet supernatant was injected into ear skin of mice with (A) and without (B) platelet depletion, without challenge. Vehicle treated with antichemokine antibodies was also injected into ear skin (C). Data are shown as percentages of basal numbers in mice injected with the supernatant of fixed platelets. Five mice per group. ∗∗P < .01. NS, Not significant. Journal of Allergy and Clinical Immunology  , e9DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

12 Effect of antiplatelet drugs on formation of platelet-leukocyte complexes and chemokine release from platelets. P-selectin expression on platelets and the number of platelet-leukocyte complexes were examined in the blood of mice (6 per group) treated with antiplatelet agents (A). Chemokine levels were measured in triplicate in the supernatant of platelets treated with antiplatelet agents (B). ∗∗P < .01. OVA, Ovalbumin. Journal of Allergy and Clinical Immunology  , e9DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Platelets play important roles in the late phase of the immediate hypersensitivity reaction  Risa Tamagawa-Mineoka, MD, PhD, Norito Katoh, MD, PhD, Saburo."

Similar presentations

CD4+CD25+ regulatory T cells suppress contact hypersensitivity reactions through a CD39, adenosine-dependent mechanism  Sabine Ring, PhD, Stephen J. Oliver,

CD4+CD25+ regulatory T cells suppress contact hypersensitivity reactions through a CD39, adenosine-dependent mechanism  Sabine Ring, PhD, Stephen J. Oliver,
Epoxyeicosatrienoic acids are involved in the C70 fullerene derivative–induced control of allergic asthma  Sarah K. Norton, PhD, Dayanjan S. Wijesinghe,

Epoxyeicosatrienoic acids are involved in the C70 fullerene derivative–induced control of allergic asthma  Sarah K. Norton, PhD, Dayanjan S. Wijesinghe,
Weiguo Chen, PhD, Umasundari Sivaprasad, PhD, Aaron M

Weiguo Chen, PhD, Umasundari Sivaprasad, PhD, Aaron M
Intravenous immunoglobulin treatment abrogates transplacental autoantibody transfer in a murine pemphigus model  Sachiko Ono, MD, PhD, Gyohei Egawa, MD,

Intravenous immunoglobulin treatment abrogates transplacental autoantibody transfer in a murine pemphigus model  Sachiko Ono, MD, PhD, Gyohei Egawa, MD,
Innate IL-13–producing nuocytes arise during allergic lung inflammation and contribute to airways hyperreactivity  Jillian L. Barlow, PhD, Agustin Bellosi,

Innate IL-13–producing nuocytes arise during allergic lung inflammation and contribute to airways hyperreactivity  Jillian L. Barlow, PhD, Agustin Bellosi,
IL-21 Reduces Immediate Hypersensitivity Reactions in Mouse Skin by Suppressing Mast Cell Activation or IgE Production  Risa Tamagawa-Mineoka, Tsunao.

IL-21 Reduces Immediate Hypersensitivity Reactions in Mouse Skin by Suppressing Mast Cell Activation or IgE Production  Risa Tamagawa-Mineoka, Tsunao.
David A. Khan, MD  Journal of Allergy and Clinical Immunology 

David A. Khan, MD  Journal of Allergy and Clinical Immunology 
Epicutaneous sensitization results in IgE-dependent intestinal mast cell expansion and food-induced anaphylaxis  Lisa M. Bartnikas, MD, Michael F. Gurish,

Epicutaneous sensitization results in IgE-dependent intestinal mast cell expansion and food-induced anaphylaxis  Lisa M. Bartnikas, MD, Michael F. Gurish,
Autocrine hemokinin-1 functions as an endogenous adjuvant for IgE-mediated mast cell inflammatory responses  Tina L. Sumpter, PhD, Chin H. Ho, MD, Anna.

Autocrine hemokinin-1 functions as an endogenous adjuvant for IgE-mediated mast cell inflammatory responses  Tina L. Sumpter, PhD, Chin H. Ho, MD, Anna.
IL-33 dysregulates regulatory T cells and impairs established immunologic tolerance in the lungs  Chien-Chang Chen, PhD, Takao Kobayashi, PhD, Koji Iijima,

IL-33 dysregulates regulatory T cells and impairs established immunologic tolerance in the lungs  Chien-Chang Chen, PhD, Takao Kobayashi, PhD, Koji Iijima,
Leukocyte nicotinamide adenine dinucleotide phosphate-reduced oxidase is required for isocyanate-induced lung inflammation  Si-Yen Liu, PhD, Wei-Zhi Wang,

Leukocyte nicotinamide adenine dinucleotide phosphate-reduced oxidase is required for isocyanate-induced lung inflammation  Si-Yen Liu, PhD, Wei-Zhi Wang,
Is 9 more than 2 also in allergic airway inflammation?

Is 9 more than 2 also in allergic airway inflammation?
Eosinophils contribute to the resolution of lung-allergic responses following repeated allergen challenge  Katsuyuki Takeda, MD, PhD, Yoshiki Shiraishi,

Eosinophils contribute to the resolution of lung-allergic responses following repeated allergen challenge  Katsuyuki Takeda, MD, PhD, Yoshiki Shiraishi,
Arabinogalactan isolated from cowshed dust extract protects mice from allergic airway inflammation and sensitization  Marcus Peters, PhD, Marion Kauth,

Arabinogalactan isolated from cowshed dust extract protects mice from allergic airway inflammation and sensitization  Marcus Peters, PhD, Marion Kauth,
Color-coded real-time cellular imaging of lung T-lymphocyte accumulation and focus formation in a mouse asthma model  Akihiro Hasegawa, PhD, Katsuhiro.

Color-coded real-time cellular imaging of lung T-lymphocyte accumulation and focus formation in a mouse asthma model  Akihiro Hasegawa, PhD, Katsuhiro.
Signaling through FcRγ-associated receptors on dendritic cells drives IL-33–dependent TH2-type responses  Melissa Y. Tjota, BA, Cara L. Hrusch, PhD, Kelly.

Signaling through FcRγ-associated receptors on dendritic cells drives IL-33–dependent TH2-type responses  Melissa Y. Tjota, BA, Cara L. Hrusch, PhD, Kelly.
Peroxisome proliferator-activated receptor α regulates skin inflammation and humoral response in atopic dermatitis  Delphine Staumont-Sallé, MD, Georges.

Peroxisome proliferator-activated receptor α regulates skin inflammation and humoral response in atopic dermatitis  Delphine Staumont-Sallé, MD, Georges.
Reply to: “A note on normality”

Reply to: “A note on normality”
Matrix metalloproteinase 12 is produced by M2 macrophages and plays important roles in the development of contact hypersensitivity  Daiki Nakagomi, MD,

Matrix metalloproteinase 12 is produced by M2 macrophages and plays important roles in the development of contact hypersensitivity  Daiki Nakagomi, MD,
Early-life chlamydial lung infection enhances allergic airways disease through age- dependent differences in immunopathology  Jay C. Horvat, PhD, Malcolm.

Early-life chlamydial lung infection enhances allergic airways disease through age- dependent differences in immunopathology  Jay C. Horvat, PhD, Malcolm.

Similar presentations

Ads by Google