1. Plant Pigment
Chromatography
Visual Instructions
Step through the slides to see how to set up your experiment.

2. Materials:
Beaker
Chromatography Paper
150ml Beaker
Spinach Leaf
Coin
SAFETY
BE SURE TO WEAR YOUR GOGGLES DURING LAB

3. Step 1:
Cut a small triangular shape into one end of the chromatography paper

4. Step 2:
Draw a line across the paper with a pencil
NOTE: Do Not Use an Ink Pen the Ink will Dissolve during the experiment

5. Step 3:
Lay the spinach leaf over the paper and roll a coin back and forth, over the line, 2-3 times
This will leave a green line of pigment on the paper.
Let the pigment dry and then repeat this process.

6. Step 4:
Make sure your goggles are on!!!
Pour a small amount of chromatography solution into your beaker. There should be just enough to cover the bottom of the beaker.

7. Step 5:
Gently place only the tip of the chromatography paper into the solution at the bottom of the beaker.
Fold the upper end of the paper over the beaker to hold it in place

8. Allow the solution to move all the way up the paper as far as it will go. It may not make it to the top
You will see different colors appearing. These are the different pigments in the leaf.
Greens are Chlorophylls
Yellows are Carotenes and Xanthophylls

9. Take your paper out of the beaker, let it dry.
Place your name/names on the paper and give it to Mr. Haug
Clean up and return all your equipment to the counter.
Be sure there are 4 pairs of Goggles on your table before you leave