1. Proteomic Serum Profile of Fatigued Men Receiving Localized External Beam Radiation Therapy for Non-Metastatic Prostate Cancer 
Nada Lukkahatai, PhD, RN, Sajni Patel, MS, Marjan Gucek, PhD, Chao-Pin Hsiao, PhD, RN, Leorey N. Saligan, PhD, RN, CRNP 
Journal of Pain and Symptom Management 
Volume 47, Issue 4, Pages e4 (April 2014)
DOI: /j.jpainsymman
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2. Fig. 1
Sypro ruby stained gel image. The Sypro ruby stained gel image illustrates numbered positions of peptides, which were identified by LC-MS/MS to detect proteins that consistently changed in the 2-D DIGE image of depleted sera collected from baseline to midpoint of EBRT. These numbers were used to identify the proteins that were detected in the gel spot. LC-MS/MS=liquid chromatography tandem mass spectrometry; 2-D DIGE=dimensional difference gel electrophoresis; EBRT=external beam radiation therapy; pI= isoelectric point; MW= molecular weight.
Journal of Pain and Symptom Management  , e4DOI: ( /j.jpainsymman )
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3. Fig. 2
Protein identification of gel spots. These proteins were found to consistently change in the 2-D DIGE image of the sera of four HF subjects collected at baseline and midpoint of external beam radiation therapy. FC= fold change; HF=high fatigue; 2-D DIGE=two-dimensional difference gel electrophoresis.
Journal of Pain and Symptom Management  , e4DOI: ( /j.jpainsymman )
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4. Fig. 3
Western Blot band intensities. Non-normalized Western blot band intensities confirming the three proteins observed to consistently change from the 2-D DIGE image of the sera of 12 subjects collected at baseline (Day 0) and midpoint (Day 21) of external beam radiation therapy. HF=high fatigue; NF=no fatigue; 2-D DIGE=two-dimensional difference gel electrophoresis.
Journal of Pain and Symptom Management  , e4DOI: ( /j.jpainsymman )
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