Presentation is loading. Please wait.

Presentation is loading. Please wait.

Sevoflurane suppresses tumour necrosis factor-α-induced inflammatory responses in small airway epithelial cells after anoxia/reoxygenation  K. Watanabe,

Published byRandell Scott Modified over 5 years ago

Similar presentations

Presentation on theme: "Sevoflurane suppresses tumour necrosis factor-α-induced inflammatory responses in small airway epithelial cells after anoxia/reoxygenation  K. Watanabe,"— Presentation transcript:

1 Sevoflurane suppresses tumour necrosis factor-α-induced inflammatory responses in small airway epithelial cells after anoxia/reoxygenation  K. Watanabe, C. Iwahara, H. Nakayama, K. Iwabuchi, T. Matsukawa, K. Yokoyama, K. Yamaguchi, Y. Kamiyama, E. Inada  British Journal of Anaesthesia  Volume 110, Issue 4, Pages (April 2013) DOI: /bja/aes469 Copyright © 2013 The Author(s) Terms and Conditions

2 Fig 1 Effects of sevoflurane on TNF-α-induced IL-6, IL-8, and MCP-1 gene expression in SAEC. SAEC were stimulated by TNF-α (0, 5, 10, 50 ng ml−1) under A/R conditions without (blue bar) or with 1.1 vol% (green bar) or 2.2 vol% (red bar) sevoflurane. Levels of IL-6 (a), IL-8 (b), and MCP-1 (c) gene expression were measured by quantitative real-time RT–PCR. The data were normalized relative to β-actin as an internal control and are presented as mean (sd); n=3 per group. *P<0.05, **P<0.01, ***P<0.001, ****P< for the comparison between A/R and A/R+sevo. British Journal of Anaesthesia  , DOI: ( /bja/aes469) Copyright © 2013 The Author(s) Terms and Conditions

3 Fig 2 Effects of sevoflurane on TNF-α (50 ng ml−1)-induced production of IL-6 and IL-8 in SAEC. Concentrations of IL-6 (a) and IL-8 (b) in cell supernatants were measured by ELISA. Data are presented as mean (sd); n=3 per group. ****P< for the comparison between A/R and A/R+sevo. British Journal of Anaesthesia  , DOI: ( /bja/aes469) Copyright © 2013 The Author(s) Terms and Conditions

4 Fig 3 Effect of sevoflurane on TNF-a-induced activation of p65 NF-κB. (a) The effect of sevoflurane on nuclear translocation of p65 NF-κB was analysed by immunoblotting. (b) Band density was measured by densitometric analysis. Data were normalized relative to lamin C as an internal control, and are presented as mean (sd) of three independent experiments. **P < 0.01 for the comparison between A/R and A/R+sevo. (c–e) Levels of NFKB1, NFKBIA, and NFKBIE gene expression were measured by quantitative real-time RT-PCR. Data were normalized relative to β-actin as an internal control. Black and white bars represent the experiments without and with sevoflurane, respectively. Data are presented as mean (sd); n = 3 per group. *P < 0.05 for the comparison between A/R and A/R+sevo. British Journal of Anaesthesia  , DOI: ( /bja/aes469) Copyright © 2013 The Author(s) Terms and Conditions

5 Fig 4 Effect of sevoflurane on mitochondrial integrity in TNF-α-stimulated SAEC. (a) SAEC were stained with JC-1 and analysed by flow cytometry. Ordinate: mitochondrial membrane potential (Δψm). CCCP, carbonyl cyanide 3-chlorophenylhydrazone (a reagent that causes rapid depolarization of Δψm). (b) To account for variations in mitochondrial volume, the ratio of FL2/FL1 shown in (a) was calculated. Each bar represents the mean (sd) of three experiments. British Journal of Anaesthesia  , DOI: ( /bja/aes469) Copyright © 2013 The Author(s) Terms and Conditions

Download ppt "Sevoflurane suppresses tumour necrosis factor-α-induced inflammatory responses in small airway epithelial cells after anoxia/reoxygenation  K. Watanabe,"

Similar presentations

Glucocorticoids Augment the Chemically Induced Production and Gene Expression of Interleukin-1α through NF-κB and AP-1 Activation in Murine Epidermal.

Glucocorticoids Augment the Chemically Induced Production and Gene Expression of Interleukin-1α through NF-κB and AP-1 Activation in Murine Epidermal.
EGCG prevents PCB-126-induced endothelial cell inflammation via epigenetic modifications of NF-κB target genes in human endothelial cells  Dandan Liu,

EGCG prevents PCB-126-induced endothelial cell inflammation via epigenetic modifications of NF-κB target genes in human endothelial cells  Dandan Liu,
Aprotinin improves kidney function and decreases tubular cell apoptosis and proapoptotic signaling after renal ischemia-reperfusion  Ajay Kher, MD, Kirstan.

Aprotinin improves kidney function and decreases tubular cell apoptosis and proapoptotic signaling after renal ischemia-reperfusion  Ajay Kher, MD, Kirstan.
Pro-inflammatory stimulation of meniscus cells increases production of matrix metalloproteinases and additional catabolic factors involved in osteoarthritis.

Pro-inflammatory stimulation of meniscus cells increases production of matrix metalloproteinases and additional catabolic factors involved in osteoarthritis.
Volume 79, Issue 11, Pages (June 2011)

Volume 79, Issue 11, Pages (June 2011)
Inflammatory Protein Serum Amyloid A1 Marks a Subset of Conventional Renal Cell Carcinomas with Fatal Outcome  Claudia Paret, Zorica Schön, Adrianna Szponar,

Inflammatory Protein Serum Amyloid A1 Marks a Subset of Conventional Renal Cell Carcinomas with Fatal Outcome  Claudia Paret, Zorica Schön, Adrianna Szponar,
Soluble BAFF-R produced by decidual stromal cells plays an inhibitory role in monocytes and macrophages  B.P. Deng, Y. Zhang, Q.J. Wang, X.F. Xu, H. Zhang,

Soluble BAFF-R produced by decidual stromal cells plays an inhibitory role in monocytes and macrophages  B.P. Deng, Y. Zhang, Q.J. Wang, X.F. Xu, H. Zhang,
Celecoxib exerts protective effects on extracellular matrix metabolism of mandibular condylar chondrocytes under excessive mechanical stress  S.-C. Su,

Celecoxib exerts protective effects on extracellular matrix metabolism of mandibular condylar chondrocytes under excessive mechanical stress  S.-C. Su,
Elastic fibers reconstructed using adenovirus-mediated expression of tropoelastin and tested in the elastase model of abdominal aortic aneurysm in rats 

Elastic fibers reconstructed using adenovirus-mediated expression of tropoelastin and tested in the elastase model of abdominal aortic aneurysm in rats 
Modulation by aspirin of nuclear phospho–signal transducer and activator of transcription 6 expression: Possible role in therapeutic benefit associated.

Modulation by aspirin of nuclear phospho–signal transducer and activator of transcription 6 expression: Possible role in therapeutic benefit associated.
Differential Regulation by IL-1β and EGF of Expression of Three Different Hyaluronan Synthases in Oral Mucosal Epithelial Cells and Fibroblasts and Dermal.

Differential Regulation by IL-1β and EGF of Expression of Three Different Hyaluronan Synthases in Oral Mucosal Epithelial Cells and Fibroblasts and Dermal.
M. -H. Moon, J. -K. Jeong, Y. -J. Lee, J. -W. Seol, C. J. Jackson, S

M. -H. Moon, J. -K. Jeong, Y. -J. Lee, J. -W. Seol, C. J. Jackson, S
Characterization of TNF-α– and IL-17A–Mediated Synergistic Induction of DEFB4 Gene Expression in Human Keratinocytes through IκBζ  Claus Johansen, Trine.

Characterization of TNF-α– and IL-17A–Mediated Synergistic Induction of DEFB4 Gene Expression in Human Keratinocytes through IκBζ  Claus Johansen, Trine.
Dual Role of Act1 in Keratinocyte Differentiation and Host Defense: TRAF3IP2 Silencing Alters Keratinocyte Differentiation and Inhibits IL-17 Responses 

Dual Role of Act1 in Keratinocyte Differentiation and Host Defense: TRAF3IP2 Silencing Alters Keratinocyte Differentiation and Inhibits IL-17 Responses 
Randomized controlled trial comparing the McGrath videolaryngoscope with the C-MAC videolaryngoscope in intubating adult patients with potential difficult.

Randomized controlled trial comparing the McGrath videolaryngoscope with the C-MAC videolaryngoscope in intubating adult patients with potential difficult.
NF-κBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1β-induced and TNF-α-induced chondrocytes  Dr C. Lianxu, Ph.D.,

NF-κBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1β-induced and TNF-α-induced chondrocytes  Dr C. Lianxu, Ph.D.,
The Protective Effects of Melittin on Propionibacterium acnes–Induced Inflammatory Responses In Vitro and In Vivo  Woo-Ram Lee, Kyung-Hyun Kim, Hyun-Jin.

The Protective Effects of Melittin on Propionibacterium acnes–Induced Inflammatory Responses In Vitro and In Vivo  Woo-Ram Lee, Kyung-Hyun Kim, Hyun-Jin.
Y. Higashimoto, M. Ohata, Y. Yamagata, T. Iwata, M. Masuda, T

Y. Higashimoto, M. Ohata, Y. Yamagata, T. Iwata, M. Masuda, T
Expression of Protease-Activated Receptor-2 in SZ95 Sebocytes and its Role in Sebaceous Lipogenesis, Inflammation, and Innate Immunity  Sang E. Lee, Ji-Min.

Expression of Protease-Activated Receptor-2 in SZ95 Sebocytes and its Role in Sebaceous Lipogenesis, Inflammation, and Innate Immunity  Sang E. Lee, Ji-Min.
Z. Y. Yu, J. Geng, Z. Q. Li, Y. B. Sun, S. L. Wang, J. Masters, D. X

Z. Y. Yu, J. Geng, Z. Q. Li, Y. B. Sun, S. L. Wang, J. Masters, D. X

Similar presentations

Ads by Google