1. The Expression of Bcl-x Is Downregulated During Differentiation of Human Hematopoietic Progenitor Cells Along the Granulocyte But Not the Monocyte/Macrophage Lineage
by Cristina Sanz, Adalberto Benito, Maite Silva, Beatriz Albella, Carlos Richard, Jose Carlos Segovia, Andres Insunza, Juan Antonio Bueren, and Jose Luis Fernández-Luna
Blood
Volume 89(9):
May 1, 1997
©1997 by American Society of Hematology

2. Cristina Sanz et al. Blood 1997;89:3199-3204
©1997 by American Society of Hematology

3. Cristina Sanz et al. Blood 1997;89:3199-3204
©1997 by American Society of Hematology

4. Expression of Bcl-x in CD34+ cells undergoing granulocyte or monocyte/macrophage differentiation.
Expression of Bcl-x in CD34+ cells undergoing granulocyte or monocyte/macrophage differentiation. Cells were cultured as described in Fig 1, and at the indicated time points, Bcl-x protein was analyzed by an immunocytochemical procedure using a rabbit antibody against human Bcl-x. After incubation with biotinilated goat antirabbit IgG and ExtrAvidin alkaline phosphatase, staining was developed using BCIP/NBT substrate (original magnification × 1,000). No signal was detectable in the negative controls. All stainings are from a representative experiment (n = 3).
Cristina Sanz et al. Blood 1997;89:
©1997 by American Society of Hematology

5. Morphology and Bcl-x expression in HL-60 cells treated with PMA or RA
Morphology and Bcl-x expression in HL-60 cells treated with PMA or RA. HL-60 cells untreated or after culturing in the presence of PMA for 4 days or RA for 6 days were cytocentrifuged and either stained with May-Grunwald-Giemsa solution or analyzed for the ...
Morphology and Bcl-x expression in HL-60 cells treated with PMA or RA. HL-60 cells untreated or after culturing in the presence of PMA for 4 days or RA for 6 days were cytocentrifuged and either stained with May-Grunwald-Giemsa solution or analyzed for the expression of Bcl-x using a rabbit anti–-Bcl-x antibody. After incubation with biotinilated goat antirabbit IgG and ExtrAvidin alkaline phosphatase, staining was developed using BCIP/NBT substrate (original magnification × 1,000). No signal was detectable in the negative controls. All stainings are from a representative experiment (n = 3).
Cristina Sanz et al. Blood 1997;89:
©1997 by American Society of Hematology

6. Flow cytometric analysis of Bcl-x and Bcl-2 in CD34+ cells undergoing granulocyte or monocyte/macrophage differentiation.
Flow cytometric analysis of Bcl-x and Bcl-2 in CD34+ cells undergoing granulocyte or monocyte/macrophage differentiation. Cells were cultured in the presence of G-CSF or M-CSF and at the indicated time points differentiating cells were stained with mouse antihuman Bcl-x, hamster antihuman Bcl-2 (6C8) or irrelevant antibody as a background control (dotted lines) followed by biotin-conjugated goat antimouse or antihamster IgG and PE-conjugated streptavidin. All histograms are from a representative experiment (n = 3).
Cristina Sanz et al. Blood 1997;89:
©1997 by American Society of Hematology

7. Expression of bcl-x mRNA in HL-60 and CD34+ cells differentiated into monocytes/macrophages.
Expression of bcl-x mRNA in HL-60 and CD34+ cells differentiated into monocytes/macrophages. Total RNA was purified from HL-60 cells treated with PMA for 4 days and CD34+ cells after 21 days of culture in the presence of M-CSF, and subjected to RT-PCR analysis with oligonucleotide primers that amplify both bcl-xL (340 bp) and bcl-xS (151 bp). Plasmids containing bcl-xL (SFFV-bcl-xL ) or bcl-xS (SFFV-bcl-xS ) cDNAs were also amplified as positive controls. After 30 cycles, PCR products were electrophoresed in a 2% agarose gel and stained with ethidium bromide.
Cristina Sanz et al. Blood 1997;89:
©1997 by American Society of Hematology