Presentation is loading. Please wait.

Presentation is loading. Please wait.

Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells Cell Physiol Biochem.

Published byWhitney Conley Modified over 5 years ago

Similar presentations

Presentation on theme: "Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells Cell Physiol Biochem."— Presentation transcript:

1 Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells Cell Physiol Biochem 2017;44:133– DOI: / Fig. 1. mRNA and protein levels of CHOP, GRP78, IRE1α, PI3K, AKT and mTOR in the mutant and wild-type p53 groups. Note: A. Diagram of protein expression levels of CHOP, GRP78, IRE1α, PI3K, AKT and mTOR detected by Western blotting; B. Relative protein expression levels of CHOP, GRP78, IRE1α, PI3K, AKT and mTOR in the mutant and wild-type p53 groups; C. Relative mRNA expression levels of CHOP, GRP78, IRE1α, PI3K, AKT and mTOR in the mutant and wild-type p53 groups; *P < 0.05, indicates a comparison with P53-wild-type group; CHOP, C/EBP homologous protein; GRP78, glucose-regulated protein 78; IRE1α, inositolrequiring enzyme-1α; PI3K, phosphatidyl inositol 3-kinase; Akt, protein kinase B; mTOR, mammalian target of rapamycin. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

2 Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells Cell Physiol Biochem 2017;44:133– DOI: / Fig. 2. mRNA and protein expression levels of CHOP, GRP78 and IRE1α in mutant p53 LC cells in each group Note: A. Protein levels of CHOP, GRP78 and IRE1α in mutant p53 LC cells detected by Western blotting; B. Protein levels of CHOP, GRP78 and IRE1α in mutant p53 H322 cells; C. mRNA expression levels of CHOP, GRP78 and IRE1α in mutant p53 H322 cells; D. Protein levels of CHOP, GRP78 and IRE1α in mutant p53 NCI-H157 cells; E. mRNA expression levels of CHOP, GRP78 and IRE1α in mutant p53 NCI-H157 cells; *P<0.05, represents a comparison with the blank group; #P<0.05, represents a comparison with the ERS group; LC, lung cancer; ERS, endoplasmic reticulum stress; CHOP, C/EBP homologous protein; GRP78, glucose-regulated protein 78; IRE1α, inositol-requiring enzyme-1α. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

3 Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells Cell Physiol Biochem 2017;44:133– DOI: / Fig. 3. mRNA and protein expression levels of PI3K, AKT and mTOR in mutant p53 LC cells in each group. Note: A. Protein levels of PI3K, AKT and mTOR in H322 and NCI-H157 cell lines detected by Western blotting; B. Relative protein expression levels of PI3K, AKT and mTOR in the mutant p53 H322 cell line; C. Relative mRNA expression levels of PI3K, AKT and mTOR in the mutant p53 H322 cell line; D. Relative protein expression of PI3K, AKT and mTOR in the mutant p53 NCI-H157 cell line; E. Relative mRNA expression of PI3K, AKT and mTOR in the mutant p53 NCI-H157 cell line; *P<0.05, represents a comparison with the blank group; #P<0.05, represents a comparison with the ERS group; LC, lung cancer; ERS, endoplasmic reticulum stress; PI3K, phosphatidyl inositol 3-kinase; Akt, protein kinase B; mTOR, mammalian target of rapamycin © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

4 Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells Cell Physiol Biochem 2017;44:133– DOI: / Fig. 4. mRNA and protein expression levels of the autophagy factors LC3-II/LC3-I, Atg5, and Atg7 in mutant p53 LC cells in each group Note: A. Protein levels of LC3-I, LC3-II, Atg5, and Atg7 in the H322 and NCI-H157 cell lines for the five groups detected by Western blotting; B. Relative protein expression levels of LC3-II/LC3-I, Atg5 and Atg7 in the mutant p53 H322 cell line for the five groups; C. Relative mRNA expression levels of LC3-II/LC3-I, Atg5, and Atg7 in the mutant p53 H322 cell line; D. Relative protein expression levels of LC3-II/LC3-I, Atg5, and Atg7 in the mutant p53 NCI-H157 cell line; E. Relative mRNA expression levels of LC3-II/LC3-I, Atg5, and Atg7 in the mutant p53 NCI-H157 cell line; *P<0.05, represents a comparison with the blank group; #P<0.05. represents a comparison with the ERS group; &P<0.05, represents a comparison with the rapamycin group; LC, lung cancer; ERS, endoplasmic reticulum stress; LC3, light chain 3; Atg5, autophagyrelated gene 5; Atg7, autophagy-related gene 7. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

5 Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells Cell Physiol Biochem 2017;44:133– DOI: / Fig. 5. Number of autophagosomes formed in mutant p53 LC cells in each group Note: Blue represents the nucleus; Green represents LC3 fusion protein-marked autophagosomes; Scale bar = 10 µm; Red arrows indicate autophagosomes; *P<0.05, represents a comparison with the blank group; #P<0.05, represents a comparison with the ERS group; &P<0.05, represents a comparison with the rapamycin group; LC, lung cancer; ERS, endoplasmic reticulum stress. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

6 Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells Cell Physiol Biochem 2017;44:133– DOI: / Fig. 6. mRNA and protein levels of caspase-3 and caspase-12 in mutant p53 LC cells in each groupNote: A. Protein levels of caspase-3, caspase-12, cleaved caspase-3 and cleaved caspase-12 in the H322 and NCI-H157 cell lines for the five groups detected by Western blotting; B. Relative protein expression levels of caspase-3, caspase-12, cleaved caspase-3 and cleaved caspase-12 in the mutant p53 H322 cell line; C. Relative mRNA expression levels of caspase-3 and caspase-12 in the mutant p53 H322 cell line; D. Relative protein expression levels of caspase-3, caspase-12, cleaved caspase-3 and cleaved caspase-12 in the mutant p53 NCI-H157 cell line; E. Relative mRNA expression levels of caspase-3 and caspase-12 in the mutant p53 NCI-H157 cell line; *P<0.05, represents a comparison with the blank group; #P<0.05, represents a comparison with the ERS group; &P<0.05, represents a comparison with the rapamycin group; LC, lung cancer; ERS, endoplasmic reticulum stress © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

7 Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells Cell Physiol Biochem 2017;44:133– DOI: / Fig. 7. Apoptosis rates in mutant p53 LC cells induced by ERS in each groupNote: A. Cell apoptosis in the mutant p53 H322 cell line for the five groups detected by flow cytometry; B. Cell apoptosis rate in the mutant p53 NCI-H157 cell line for the five groups; * P < , represents a comparison with the blank group; #P<0.05, represents a comparison with the ERS group; & represents a comparison with the rapamycin group; LC, lung cancer; ERS, endoplasmic reticulum stress. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

8 Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells Cell Physiol Biochem 2017;44:133– DOI: / Fig. 8. Proliferation in mutant p53 LC cells induced by ERS in each groupNote: A. Cell proliferation in the mutant p53 H322 cell line detected by BrdU cell proliferation assay; Scale bar = 20 um; B. Cell proliferation in the mutant p53 NCI-H157 cell line detected by BrdU cell proliferation assay; C: Statistical analysis of BrdU-positive H1322 cells; D: Statistical analysis of BrdU-positive NCI-H157 cells; *P<0.05, represents a comparison with the blank group; #P<0.05, represents a comparison with the ERS group; BrdU, 5’bromo-2’deoxyuridine; LC, lung cancer; ERS, endoplasmic reticulum stress. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

Download ppt "Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells Cell Physiol Biochem."

Similar presentations

Overexpression of endoplasmic reticulum-resident chaperone attenuates cardiomyocyte death induced by proteasome inhibition by Hai Ying Fu, Tetsuo Minamino,

Overexpression of endoplasmic reticulum-resident chaperone attenuates cardiomyocyte death induced by proteasome inhibition by Hai Ying Fu, Tetsuo Minamino,
Cell Physiol Biochem 2017;42:2582– DOI: /

Cell Physiol Biochem 2017;42:2582– DOI: /
Comparative Profiling of Triple-Negative Breast Carcinomas Tissue Glycoproteome by Sequential Purification of Glycoproteins and Stable Isotope Labeling.

Comparative Profiling of Triple-Negative Breast Carcinomas Tissue Glycoproteome by Sequential Purification of Glycoproteins and Stable Isotope Labeling.
TNF-α- Mediated-p38-Dependent Signaling Pathway Contributes to Myocyte Apoptosis in Rats Subjected to Surgical Trauma Cell Physiol Biochem 2015;35:1454-1466.

TNF-α- Mediated-p38-Dependent Signaling Pathway Contributes to Myocyte Apoptosis in Rats Subjected to Surgical Trauma Cell Physiol Biochem 2015;35:
Leukotriene B4 Inhibits L-Type Calcium Channels via p38 Signaling Pathway in Vascular Smooth Muscle Cells Cell Physiol Biochem 2015;37:1903-1913.

Leukotriene B4 Inhibits L-Type Calcium Channels via p38 Signaling Pathway in Vascular Smooth Muscle Cells Cell Physiol Biochem 2015;37:
Cell Physiol Biochem 2014;33: DOI: /

Cell Physiol Biochem 2014;33: DOI: /
Inducible EGFR T790M-Mediated Gefitinib Resistance in Non-small Cell Lung Cancer Cells Does Not Modulate Sensitivity to PI103 Provoked Autophagy  Flavia.

Inducible EGFR T790M-Mediated Gefitinib Resistance in Non-small Cell Lung Cancer Cells Does Not Modulate Sensitivity to PI103 Provoked Autophagy  Flavia.
Nasal Cavity Paraganglioma: Literature Review and Discussion of a Rare Case Biomed Hub 2017;2:464099 - DOI:10.1159/000464099 © 2017 The Author(s) Published.

Nasal Cavity Paraganglioma: Literature Review and Discussion of a Rare Case Biomed Hub 2017;2: DOI: / © 2017 The Author(s) Published.
Case Rep Oncol 2017;10:361– DOI: /

Case Rep Oncol 2017;10:361– DOI: /
OPN -443C>T Genetic Polymorphism and Tumor OPN Expression are Associated with the Risk and Clinical Features of Papillary Thyroid Cancer in a Chinese Cohort.

OPN -443C>T Genetic Polymorphism and Tumor OPN Expression are Associated with the Risk and Clinical Features of Papillary Thyroid Cancer in a Chinese Cohort.
Cell Physiol Biochem 2013;32: DOI: /

Cell Physiol Biochem 2013;32: DOI: /
Cell Physiol Biochem 2016;38: DOI: /

Cell Physiol Biochem 2016;38: DOI: /
Astragaloside IV Enhances Cisplatin Chemosensitivity in Non-Small Cell Lung Cancer Cells Through Inhibition of B7-H3 Cell Physiol Biochem 2016;40:1221-1229.

Astragaloside IV Enhances Cisplatin Chemosensitivity in Non-Small Cell Lung Cancer Cells Through Inhibition of B7-H3 Cell Physiol Biochem 2016;40:
Extracellular HSP70 Activates ERK1/2, NF-kB and Pro-Inflammatory Gene Transcription Through Binding with RAGE in A549 Human Lung Cancer Cells Cell Physiol.

Extracellular HSP70 Activates ERK1/2, NF-kB and Pro-Inflammatory Gene Transcription Through Binding with RAGE in A549 Human Lung Cancer Cells Cell Physiol.
A Novel Cinnamide YLT26 Induces Breast Cancer Cells Apoptosis via ROS-Mitochondrial Apoptotic Pathway in Vitro and Inhibits.

A Novel Cinnamide YLT26 Induces Breast Cancer Cells Apoptosis via ROS-Mitochondrial Apoptotic Pathway in Vitro and Inhibits.
Effect of Shikonin on Spinal Cord Injury in Rats Via Regulation of HMGB1/TLR4/NF-kB Signaling Pathway Cell Physiol Biochem 2017;43:481–491 - DOI:10.1159/000480474.

Effect of Shikonin on Spinal Cord Injury in Rats Via Regulation of HMGB1/TLR4/NF-kB Signaling Pathway Cell Physiol Biochem 2017;43:481–491 - DOI: /
AntimiR-30b Inhibits TNF-α Mediated Apoptosis and Attenuated Cartilage Degradation through Enhancing Autophagy Cell Physiol Biochem 2016;40:883-894 -

AntimiR-30b Inhibits TNF-α Mediated Apoptosis and Attenuated Cartilage Degradation through Enhancing Autophagy Cell Physiol Biochem 2016;40:
Effects of Glycyrrhizin in a Mouse Model of Lung Adenocarcinoma

Effects of Glycyrrhizin in a Mouse Model of Lung Adenocarcinoma
Cell Physiol Biochem 2015;37: DOI: /

Cell Physiol Biochem 2015;37: DOI: /
Cell Physiol Biochem 2016;38: DOI: /

Cell Physiol Biochem 2016;38: DOI: /

Similar presentations

Ads by Google