Download presentation
Presentation is loading. Please wait.
Published byΛυσιστράτη Αγγελοπούλου Modified over 6 years ago
1
Designs of adipocyte-specific binding microgel for fat reduction
대학원 세미나 Designs of adipocyte-specific binding microgel for fat reduction Keum-Yong Seong Supervised by Prof. Seung Yun Yang Functional Soft Nanomaterials Lab.
2
High-intensity focused ultrasound (HIFU)
Physical therapeutics for fat reduction High-intensity focused ultrasound (HIFU) Lasers Radiofrequency (RF) Selectively absorption in certain parts of the tissue, depending on color and penetration depth Ablative lasers, Fractional CO2 lasers Inducement of complete coagulation necrosis of a targeted tissues Need of imaging system for safe applications Temp.: oC A form of electromagnetic energy (300 MHz – 3 KHz) for selective thermolysis (inducement of apoptosis in fat tissues) Temp.: 39 – 43 oC K. Fritz et al., Facial Plast. Surg., 32, 2016 Functional Soft Nanomaterials Lab.
3
PPC injection for fat reduction
Phosphatidylcholine (PPC) and deoxycholate (DCA) compound for efficiently reducing fat tissue PPC : lecithin, main constituent of beans (a class of phospholipids) Side effects Skin ulcer Bruise DCA : a detergent for dissolution of PPC (one of the secondary bile acid) Deoxycholate induces the DNA damage. DNA damage can give rise to cancer by causing mutations. Excessive administration of PPC can destroy the muscle and blood vessels. Functional Soft Nanomaterials Lab.
4
Lipolytic drugs Caffeine Isoproterenol Mechanism of lipolysis
induced by lipolytic drugs Lipolytic drug Isoproterenol Functional Soft Nanomaterials Lab.
5
Mechanism for lipolysis
Polymeric carriers for lipolytic drugs Concept Mechanism for lipolysis Features Nanoparticles (NPs) Drug loaded NPs (liposome, nanosphere, micells…) can transport into the adipocyte by endocytosis. Particle size : < 100 nm In situ hydrogel Sustained drug delivery from 3D network structure of hydrogel Binding of released drug to β-receptor of adipocyte Lipolysis Lipolytic drug Development of carriers binding to adipocytes is required to increase the efficiency of drug delivery for lipolysis. Functional Soft Nanomaterials Lab.
6
Adipocyte-specific binding microgel
Approach for carriers to delivery the lipolytic drug Criteria for carriers to delivery the lipolytic drug High biocompatibility Large contact area between adipocytes and carriers High adhesion between adipocyte and carriers Sustained drug delivery for maintaining the therapeutic levels Adipocyte Adipocyte-specific binding microgel Designs Biopolymer based carriers Spherical particles < sheet-type particles Targeting of adipocyte-specific site Hydrogel for sustained drug delivery MICROGEL Functional Soft Nanomaterials Lab.
7
1. Candidates materials for fat reduction 2. Fabrication of microgel
3. Method for adipocyte-specific binding Functional Soft Nanomaterials Lab.
8
Chitosan as a fat binder
Chitosan as a candidate to inhibit adipogenesis Chitosan Chitosan as a fat binder Natural polymer made by treating the chitin shells of shrimp or crap with an alkaline substance (deacetylation) Properties High biocompatibility & biodegradability Muco-adhesion Chitosan carries a positive charge on the acetyl remnants and, when solubilized in an acid environment. the chitosan can bind to negatively charged molecules such as fats and lipids. Stomach (pH 2~5) Functional Soft Nanomaterials Lab.
9
Chitosan as a candidate to inhibit adipogenesis
Anti-obesity effect of chitosan in 3T3-L1 adipocyte Lipolysis Adipogenesis Significantly decreased triglyceride accumulation Inhibited PPARγ, C/EBPα by chitosan treatment inhibition of adipogenesis E. J. Cho et al., J. Microbiol. Biotechnol., 18, (2008) Functional Soft Nanomaterials Lab.
10
Extracellular matrix components for fat reduction
Extracellular matrix (ECM) Proteoglycans Collagen Reported that ingestion of diets containing gelatin or collagen protein affects the blood levels of triglycerides (TG) and/or total cholesterol (TC) in rats (Saito et al., 2009), mice (Oliveira et al., 2001), and rabbits (Terpstra et al., 1983). Functional Soft Nanomaterials Lab.
11
Chondroitin sulfate (CS)
Extracellular matrix components for fat reduction Hyaluronic acid (HA) Chondroitin sulfate (CS) Mw of HA : 2000 kDa HA was shown to regulate adipogenesis through reduction of the expression of genes such as C/EBPα and PPARγ, leading to down-regulation of SREBP-1c and FASN expression in 3T3-L1 cells and finally reduced lipid accumulation. Mw of CS : 90 kDa CS inhibited activity of pancreatic lipase. inhibition of absorption of fatty acids and monoglycerides (fatty acids and monoglycerides are formed by hydrolysis of pancreatic lipase) B.-G. Park et al., Biochem. Biophysi. Res. Commun., 467, (2015) L.-K. Han et al., Int. J. Obesity, 24, (2000) Functional Soft Nanomaterials Lab.
12
M. C. Gannon et al., Am. J. Clin. Nutr., 76, 2002
Gelatin as a candidate to induce lipolysis Gelatin A natural biopolymer derived from collagens Gelatin (Mw: K) Heating (denaturation) Collagen (Mw: 300K) Glycine treated group Glycine Gelatin consists of 30% glycine residues. Glycine can induce lipolysis as increased glucagon concentration in blood. M. C. Gannon et al., Am. J. Clin. Nutr., 76, 2002 Functional Soft Nanomaterials Lab.
13
1. Candidates materials for fat reduction 2. Fabrication of microgel
3. Method for adipocyte-specific binding Functional Soft Nanomaterials Lab.
14
Microtransfer molding method
Microgels fabricated by microtransfer molding method Microtransfer molding method Coating of hydrogel precursor on the PDMS mold after degassing in a vacuum chamber Scraping away excess material Pressing the agarose slab containing the gelling agent Floating G. T. Franzesi et al., J. Am. Chem. Soc., 128, (2006) Functional Soft Nanomaterials Lab.
15
Microgels fabricated by stop flow lithography
UV source on Mask Hydrogel precursor Hydrogel Device Flow UV source off Mask Hydrogel precursor Hydrogel Device Flow K. W. Bong et al., Langmuir, 31, (2015) Functional Soft Nanomaterials Lab.
16
1. Candidates materials for fat reduction 2. Fabrication of microgel
3. Method for adipocyte-specific binding Functional Soft Nanomaterials Lab.
17
Modification adipocyte specific binding site
Adipocyte specific binding system Functional Soft Nanomaterials Lab.
18
Thank you for your attention!
Similar presentations
© 2025 SlidePlayer.com Inc.
All rights reserved.