1. N.I Sesina*, V.R. Aleksandrova, K.V. Krasnopolskaya
IVF Programs using Embryos obtained from Vitrified and Native Donor Oocyte
N.I Sesina*,
V.R. Aleksandrova,
K.V. Krasnopolskaya
PRIOR Clinic
International Clinic”Family”
Moscow Regional Institute of Obstetrics and Gynecology
RUSSIA MOSCOW

2. Evolution of reproductive life
century
ХХI
century
35 years
Early marriage. Repeated pregnancies, childbirth, lactation →
Prolonged amenorrhoea (during life not more than 40 menstrual cycles) →
Low probability of gynecological diseases
Conscious rejection of pregnancy ("social infertility") →
Increase in the number of menstrual cycles in dozens of times (> 400 menstruation) →
Increase in the frequency of gynecological diseases

3. Pregnancy rate in IVF programs depending on age
(Prior Clinic 2016)

4. The frequency of pregnancy on the transfer of embryos Frequency of live births
≤35
35-37
38-40
≥41
Frequency of pregnancy, %
46,7%
37,8%
29,7%
19,8%
Frequency of live births, %
40,7%
31,3%
22,2%
11,8%
ASRM, 2014

5. Patients interested in cryopreservation of oocytes
Patients before surgery on the ovaries
Patients before gonadotoxic therapy
If the patient wishes to preserve her genetic material (in the absence of a sexual partner and planning a pregnancy at a later age

6. Indications for use of donor oocytes
Absence of oocytes due to natural menopause
Ovarian depletion syndrome
The condition after ovariectomy, radio or chemotherapy,
Sheriszewski-Turner Syndrome
Low quality oocytes Unsuccessful repeated attempts of IVF with insufficient ovarian response to induction of superovulation (poor response)
Repeated reception of embryos of poor quality
Multiple undeveloped pregnancies
Genetic pathologies making it impossible to have one's own healthy child

7. Strategies for donor programs
Оocyte
Vitrification
Оocyte thawing
Fertilization
Embryo transfer
Embryo cultivation
Embryo cryopreservation

8. The results of the IVF-OD program.
Vitrified DO
Thawing embryo from DO
Fresh DO
Cycles 42
101
188 ET 36 94
169
Pregnancies 9 69
Pregnancy rate
25%
40,8%
44,6%
Number of transferred embryos 78
186
405
Sacs 14 47 92
Implantation rate (on embryo)
17,9%
25,3%
22,71%

9. Program IVF-OD with vitrified oocytes
100%
90,4%
63,2%
25,4%

10. Reproductive losses
11%
14,3%
no statistically significant differences

11. Сonclusions
There are no statistically significant differences in the efficacy of IVF between groups of patients using for the fertilization native oocytes.
The use of vitrified oocytes for the fertilization in donor programs significantly reduces the effectiveness of the IVF program.
To achieve an increase in the effectiveness of IVF with vitrified donor oocytes is possible with an increase in the number of fertilized oocytes

12. Vitrified donor oocyte???
BANK OF DONOR OOCYTE
1)Opportunities for the selection of a donor
2) No synchronization needed
3) More simple mangement
4) The possibility of transporting oocytes

13. In Germany, Norway, Sweden, programs with donors of oocytes are prohibited
In Israel, the United Kingdom, Canada, programs with gratuitous donation of oocytes are allowed
In Spain, Portugal, Greece, Belgium, Finland and Russia there are no bans, but these programs are not financed by the state, so they are possible both altruistically and on a commercial basis.

14. Thank you for attention