1. Zika Virus Pathogenic Viral Infection
First observed in Zika Forest of Uganda in 1947
Infection in adult is mostly asymptomatic (1 in 5)
headache, macular/maculopapular pruritic rash, arthralgias, and conjunctivitis.
Sever congenital microcephaly of newborn
Caused by infection by the Zika Flavivirus
Typically transmitted by mosquito, primarily Aedes aegypti
Blood transfusion, sexual transmission, perinatal transmission
No Treatments or Vaccine (under development)

2. Team Workshop 1: List the experimental steps and reagents used to obtain the data above and the conclusions drawn?

3. FLAVIVIRUS
Yellow Fever
Dengue Fever
Japanese encephalitis West Nile viruses
Zika virus

4. Can you predict regions potentially important for receptor binding?
Team Workshop 1: Use online tools to obtain the protein sequence for Zika and Dengue Envelope E protein. Calculate a protein sequence similarity alignment between Zika Virus E protein and Dengue Virus E protein.
Can you predict regions potentially important for receptor binding?
Can you provide support for your prediction?
Tim and Tam phosphotidyl serine receptors.

5. Team Workshop 3: Drug Discovery
Use the RCSB to obtain the PDB file of zika virus
View the Structure in Chimera and Select the E glycoprotein subunit only.
Save as a new PDB file. Make sure it only contains the atomic coordinates for the E glycoprotein and only protein atoms. Open this file in Chimera and perform DockPrep and when finished save as a new pdb file.
Download the MOl2 file for ivermectin (IVM)
Run SwissDock (one person from Group) with the Zika glycoprotein and ivermectin.
4ONR

6. How to find it there

7. Team Workshop 4: Use STICH to investigate Ivermectin MOA.
4ONR

8. Remember Before 12 PM of the next class day:
go to b.socrative.com/student/login and complete the quiz