1. Photo credit: Russell Virgilio

2. ASTROBIOLOGY LIFE IN THE UNIVERSE Goals for today Module 1: Microscopy - acid - neutral - alkaline Module 2: Cultures - acid - neutral - alkaline

3. ASTROBIOLOGY LIFE IN THE UNIVERSE 1. Microscopy Classification

4. ASTROBIOLOGY LIFE IN THE UNIVERSE 3 Domains of Life Prokaryotes Eukaryotes DNA 1. Microscopy

5. ASTROBIOLOGY LIFE IN THE UNIVERSE Genus Cyanidia (acid-tolerant red algae) pH 0.2 – 4.0 40 – 56°C 1. Microscopy

6. Some microscopy examples from my research White Creek, Yellowstone National Park July 2012 Cyanobacteria Genus Mastigocladus

7. Some microscopy examples from my research Cyanobacteria Genus Mastigocladus

8. Some microscopy examples from my research Chocolate Pots hot springs Yellowstone National Park Cyanobacteria Genus Synechococcus

9. Some microscopy examples from my research Cyanobacteria Genus Synechococcus

10. Some microscopy examples from my research Cyanobacteria Genus Oscillatoria

11. Some microscopy examples from my research Minerals – halite (salt) gypsum Cyanobacteria Genus Spirulina Cyanobacteria Genus Spirulina

12. Some microscopy examples from my research Minerals – gypsum

13. ASTROBIOLOGY LIFE IN THE UNIVERSE Module 1: Classification Module 2: Metabolism - photosynthesis - chemosynthesis Module 3: Communities 2. Cultures

14. ASTROBIOLOGY LIFE IN THE UNIVERSE All organisms Chemotroph Phototroph Chemolithotroph Chemoorganotroph Chemical energyLight energy 2. Cultures

15. ASTROBIOLOGY LIFE IN THE UNIVERSE All organisms Chemotroph PhototrophPhototroph Chemical energyLight energy Alkaline Group 2. Cultures

16. All organisms Chemotroph PhototrophPhototroph Chemical energyLight energy Alkaline Group Phototrophs Chemotrophs

17. All organisms Chemotroph PhototrophPhototroph Chemical energyLight energy Acid Group

18. All organisms Chemotroph PhototrophPhototroph Chemical energyLight energy Acid Group - phototrophs Acid-tolerant Eukaryotic algae Chemotrophs

19. All organisms Chemotroph PhototrophPhototroph Chemical energyLight energy Neutral Group - phototrophs - chemotrophs

20. ASTROBIOLOGY LIFE IN THE UNIVERSE If youre a microbe, what do you need to eat to live? All organisms Chemotroph Phototroph Chemolithotroph Chemoorganotroph Chemical energyLight energy 1.Source of carbon 2.Source of food (electrons) 3.Source of air (oxidants) 4.Trace nutrients 2. Cultures

21. ASTROBIOLOGY LIFE IN THE UNIVERSE 2. Cultures Mg Fe Ca Mg Fe Ca Mg Fe Ca Fe Mg Ca Fe Ca Mg Fe

22. ASTROBIOLOGY LIFE IN THE UNIVERSE Goals for today Module 1: Microscopy - acid - neutral - alkaline Module 2: Cultures - acid - neutral - alkaline