1. Use and Application in Aquatic Feeds

2. Aquaculture Industry: Protein Ingredients
Need for alternative protein ingredients for the aquaculture industry
ADM is committed to providing solutions to the aquaculture industry.
PROPLEX DY is a viable, renewable plant-based protein.
Objective: present in vivo results of PROPLEX DY in various aquatic species
Amino Acid Profile
Product Stability
Consistent Nutrient Profile
Palatability
Ease of Manufacture
Extrusion/Pellet
Reduce Reliance on Fish meal

3. Product Advantages Cost effective source of amino acids.
Contains no animal proteins.
Contains no antibiotics.
Contains no known anti-nutritional factors.
Dedicated manufacturing process for consistent nutritional quality.
Excellent palatability.
PROPLEX DY is a cost-competitive, palatable, high-quality source of digestible amino acids from a yeast-based protein. It consists of dried, inactive (killed) yeast (Saccharomyces cerevisiae).

4. PROPLEX DY Production: A process from corn wet milling
Steeping
Germ (excess oil)
CORN
Germ Removal
Mycotoxin tested
Corn Gluten Feed Pellets
for
Cattle Feed
Pericarp (excess fiber)
Fiber Separation
Corn Gluten Meal
Gluten (lutein, zeaxanthin)
Gluten Separation
Starch
Fermentation and Distillation
Liquids
Evaporator
CO2
Ethanol
Human Food Products (eg. HFCS)

5. PROPLEX DY in Aquaculture
Amino Acid Digestibility
Growth Performance Tests
Proven in:
Swine
Poultry
Aquaculture
Shrimp
Salmon
Trout
Hybrid Striped Bass
Tilapia
Red Drum

6. Fish meal, Menhaden Select1
A.A. Digestibility of PROPLEX DY Across Species
46% CP
Composition
Fish meal, Menhaden Select1
PROPLEX DY
Trout
Poultry
Swine
Arginine, %
3.82 (75.5)
2.21 (81)
2.37 (92)
1.89 (79)
Histidine, %
1.08 (65.5)
1.04 (79)
1.01 (86)
0.98 (85)
Isoleucine, %
1.84 (70.4)
2.04 (79)
2.16 (90)
2.19 (85)
Leucine, %
3.38 (71.6)
5.91 (84)
4.78 (92)
5.58(90)
Lysine, %
4.09 (70.3)
2.34 (76)
2.38 (84)
2.16 (81)
Methionine, %
1.22 (80.5)
0.99 (88)
0.85 (93)
1.03 (90)
Phenylalanine, %
2.05 (67.3)
2.83 (89)
2.37 (91)
2.46 (89)
Threonine, %
2.31 (64.5)
2.15 (71)
1.96 (83)
1.77 (82)
Tryptophan, %
0.59
0.30 (91)
0.39 (89)
Valine, %
2.02 (70.4)
2.18 (79)
2.64 (88)
2.67 (86)
( ) = digestibility as measured in precision-fed rooster assay, trout or swine trial; 1trout trial

7. Hybrid Striped Bass using PROPLEX DY to reduce FM
Juvenile bass (~16g)
10 bass/tank
5 tanks per treatment
Trial length: 45 days
Ingredient
CON
(30%FM)
22.5% FM
15%
7.5% 0%
PROPLEX DY
0.0
13.5
27.0
41.3
50.7
Menhaden fish meal
30.0
22.5
15.0
7.5
Wheat Bran
23.3
17.3
11.3
4.5
0.4
RESULT
PROPLEX DY substituted up to 75% of fish meal in diets with no detriment to performance a a a a a b b b b b
Gause and Trushenski (2011) North American Journal of Aquaculture, 73:2,97-103

8. Trout Performance with PROPLEX up to 15%
Ingredient (%)
8% DY
11% DY
15% DY
PROPLEX DY
7.6
11.2
14.9
Menhaden fish meal
25.0
18.8
15.6
12.5
Wheat flour
14.5
10.5
9.5
8.3
Rainbow trout (~22g)
9 week trial
30 fish/tank
RESULT
PROPLEX DY substituted up to 38% of FM in diets (11% of diet as PROPLEX DY) with no detriment to performance
Hauptmann et. al., Evaluation of grain distillers dried yeast as a fish meal substitute in practical-type diets of juvenile rainbow trout, Oncorhynchus mykiss. Aquaculture. 432:7

9. Trout Performance with PROPLEX up to 18%
Ingredient (%)
6% DY
9% DY
12% DY
15% DY
18% DY
PROPLEX DY 6 9 12 15 18
Menhaden fish meal 20
Poultry by-product meal
15.5
13.6
12.65
11.70
10.77
9.80
Wheat flour
14.8
13.77
13.34
12.78
12.22
11.74
Rainbow trout (~22g)
9 week trial
30 fish/tank
KEY POINT
PROPLEX DY included up to 18% in trout diets supports performance
Sealey et. al., Refining inclusion levels of grain distillers dried yeast in commercial-type and plant-based diets for juvenile rainbow trout, Oncorhynchus mykiss. Journal of the World Aquaculture Society. 46:4

10. Shrimp – PROPLEX DY Can Replace Soybean Meal
Ingredient (g kg–1, as-is) DY
50 DY
100 DY
150 DY
200
Soybean meal
523.0
474.8
426.7
378.5
330.2
PROPLEX DY
0.0
50.0
100.0
150.0
200.0
Menhaden fish oil
54.9
53.9
52.9
51.9
50.9
Shrimp weight (g)
56 d feeding trial
PROPLEX DY
Achupallas, J., Y. Zhou, D. Davis Aquaculture Nutrition. 22:1222

11. Shrimp – Trial Pond Results
PROPLEX DY performs well in
real-world conditions
Shrimp weight (g)
PROPLEX DY
Achupallas, J., Y. Zhou, D. Davis Aquaculture Nutrition. 22:1222
Photo: Trial location - Claude Peteet Mariculture Center, Gulf Shores, Alabama

12. Salmon diets contained up to 15% PROPLEX DY
Ingredient, %
0% DY
5% DY
10% DY
15% DY
Poultry meal, IDF
27.5
22.5
Fish oil, menhaden 21
Sardine meal, EWOS 2011 20 15 10
Wheat flour
PROPLEX DY 5
Soy protein concentrate
9.5
Wheat gluten meal
Vitamin premix ARS 702 1
lysine-HCl
Choline CL
0.6
Stay-C
0.2
Trace mineral premix
0.1
Astaxanthin
PROPLEX DY replaced sardine meal and poultry meal.
Experimental diets were provided for days.
G. Burr, USDA-ARS Maine. Atlantic Salmon, Salmo salar

13. Salmon diets contained up to 15% PROPLEX DY
FCR
Final BW, g
Weight Gain, g
120 day test
P = 0.56
P = 0.54
P = 0.54
G. Burr, USDA-ARS Maine. Atlantic Salmon, Salmo salar

14. Recommended feeding levels
PROPLEX DY Summary
A reliable protein source with a guarantee of 48% CP
Palatable ingredient for Aquaculture
Highly digestible amino acids
Produced using wet milling technologies
Species
Recommended feeding levels
Salmon
Up to 10%
Trout
Hybrid striped bass
Shrimp

15. Citations
Achupallas, J., Y. Zhou, D. Davis Pond production of Pacific white shrimp, Litopenaeus vannamei, fed grain distillers dried yeast. Aquaculture Nutrition. 22:1222
Gause and Trushenski Production performance and stress tolerance of sunshine bass raised on reduced fish meal feeds containing ethanol yeast. North American Journal of Aquaculture. 73:168
Gause and Trushenski Replacement of fish meal with ethanol yeast in the diets of sunshine bass. North American Journal of Aquaculture. 73(2):97
Gause and Trushenski Comparative value of fish meal alternatives as protein sources in feeds for Hybrid Striped Bass. Aquaculture. 75(3):329
Hauptmann, B., F. Barrows, S. Block, T. Gaylord, J. Paterson, S. Rawles, W. Sealey Evaluation of grain distillers dried yeast as a fish meal substitute in practical-type diets of juvenile rainbow trout, Oncorhynchus mykiss. Aquaculture. 432:7
Hauptman B., F. Barrows, S. Block, T. Gaylord, J. Paterson, W. Sealey Potential for a mycotoxin deactivator to improve growth and performance of rainbow trout fed high levels of an ethanol industry co-product, grain distillers dried yeast. North American Journal of Aquaculture. 76(4):297
Kanczuzewski and Trushenski Evaluation of hydrogenated soybean oil in feeds for hybrid striped bass fed in conjunction with finishing periods of different durations. North American Journal of Aquaculture 77:8
Sealey, W., T. O’Neill, J. Peach, T. G. Gaylord, F. Barrows, S. Block Refining Inclusion Levels of Grain Distiller’s Dried Yeast in Commercial-type and Plant-based Diets for Juvenile Rainbow Trout, Oncorhynchus mykiss. Journal of the World Aquaculture Society. 46(4):434
Omolola, B., C. Yeoman, T. Gaylord, G. Duff, T. Hamerly, B. Bothner, S. Block, W. Sealey Differences in amino acid catabolism by gut microbes with/without prebiotics inclusion in GDDY-based diet affect feed utilization in rainbow trout. Aquaculture. In press.
Rosales, M., S. Castillo, C. Pohlenz, D. Gatlin Evaluation of dried yeast and threonine fermentation biomass as partial fish meal replacements in the diet of red drum Sciaenops ocellatus. Animal Feed Science and Technology. 232:190