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Finding Genes to Clone Working hypothesis: find way to alter metabolism to increase investment in biofuels
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Finding Genes to Clone Working hypothesis: find way to alter metabolism to increase investment in biofuels Increase production of photosynthate so will store some Increase Calvin cycle Increase light reactions
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Finding Genes to Clone Working hypothesis: find way to alter metabolism to increase investment in biofuels Increase production of photosynthate so will store some Increase Calvin cycle Increase light reactions Alter allocation of photosynthate
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Finding Genes to Clone Working hypothesis: find way to alter metabolism to increase investment in biofuels Increase production of photosynthate so will store some Increase Calvin cycle Increase light reactions Alter allocation of photosynthate Alter N or S uptake
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Finding Genes to Clone Working hypothesis: find way to alter metabolism to increase investment in biofuels Increase production of photosynthate so will store some Increase Calvin cycle Increase light reactions Alter allocation of photosynthate Alter N or S uptake Alter N or S assimilation
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Finding Genes to Clone Working hypothesis: find way to alter metabolism to increase investment in biofuels Increase production of photosynthate so will store some Increase Calvin cycle Increase light reactions Alter allocation of photosynthate Alter N or S uptake Alter N or S assimilation Alter biochemical pathways Push more into lipid synthesis
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Finding Genes to Clone Working hypothesis: find way to alter metabolism to increase investment in biofuels Increase production of photosynthate so will store some Increase Calvin cycle Increase light reactions Alter allocation of photosynthate Alter N or S uptake Alter N or S assimilation Alter biochemical pathways Push more into lipid synthesis Alter free fatty acid recycling
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Finding Genes to Clone Use bioinformatics to study pathways for targets KEGG: Kyoto Encyclopedia of Genes and Genomes
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Finding Genes to Clone Use bioinformatics to study pathways for targets KEGG
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Finding Genes to Clone Use bioinformatics KEGG
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Finding Genes to Clone Use bioinformatics to study pathways for targets KEGG
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caccATGCCAGACGATTACGGGACG
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caccATGCCAGACGATTACGGGACG
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caccATGCCAGACGATTACGGGACGxxxxxxxxxxxxxxxxxx
CAGGGGCGATCGGCC>TAGGACCGAAAGGCCGCTAGGTGCTGCCTCTCGCCACCACGGAT
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Plant Respiration Recovers energy stored by photosynthesis C6H12O6 + 6 O2 <=> 6 CO2 + 6 H2O + energy Occurs in all plant tissues: even source leaves in light! Releases 50% of fixed CO2
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Plant Respiration Releases 50% of fixed CO2 Provides energy for all sinks, source leaves at night & helps source during day!
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Plant Respiration Similar, but more complex than in animals Making precursors, recycling products, releasing energy are also important
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Plant Respiration Glycolysis in cytosol Pyruvate oxidation in mito Krebs cycle in mito Electron transport & chemiosmosis in mito
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Plant Respiration Glycolysis in cytosol 1 glucose -> 2 pyruvate Yields 2 NADH & 2 ATP per glucose Unique features in plants May start with DHAP from cp instead of glucose
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Unique features in plants
May start with DHAP from cp instead of glucose May yield malate cf pyr PEP ->OAA by PEPC, then reduced to malate
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Plant Respiration May yield malate cf pyr PEP ->OAA by PEPC, then reduced to malate Get more ATP/NADH in mito
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Unique features in plants
May yield malate cf pyr PEP ->OAA by PEPC, then reduced to malate Get more ATP/NADH in mito Replaces substrates
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Plant Respiration Glycolysis in cytosol 1 glucose -> 2 pyruvate Yields 2 NADH & 2 ATP per glucose Anaerobic plants ferment pyr to regenerate NAD+ Form EtOH
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Plant Respiration Glycolysis in cytosol 1 glucose -> 2 pyruvate Yields 2 NADH & 2 ATP per glucose Anaerobic plants ferment pyr to regenerate NAD+ Form EtOH Less toxic than lactate because diffuses away
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Plant Respiration Krebs cycle Similar, but more complex Key role is making intermediates & recycling products
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Plant Respiration Krebs cycle Similar, but more complex Key role is making intermediates & recycling products Many ways to feed in other substrates to burn
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Plant Respiration Krebs cycle Similar, but more complex Key role is making intermediates & recycling products Many ways to feed in other substrates to burn or replace intermediates used for biosynthesis
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