1. Template of Poster for ICTAP and SFN CONFERENCES, SEPTEMBER 20-21, 2018
Saronom Silaban1,2 , D. Sofian Wildan1, Siti Nur Inayah1, Sutarya Enus3, Iman P. Maksum1, Khomaini Hasan4, Toto Subroto1*, and Soetijoso Soemitro1
1Postgraduate Program, Universitas Negeri Medan, Medan, Indonesia,2Department of Chemistry Education, Universitas Negeri Jakarta, Jakarta, Indonesia, 3...etc.
*Corresponding authors:
This template was designed to produce a 594 mm x 841mm (A1 size) poster in portrait or landscape orientation. We’ve put in the headings we usually see in these posters, you can copy and paste and change to your hearts content! We suggest you use keep black text against a light background so that it is easy to read. Background color can be changed in format-background-drop down menu.
Abstract
Method
Background
Conclusions
Acknowledgments
References
Soejima et al J Biochem. 130,
Strocchi et al Proteomics. 6,
Walter and Buchner Angew. Chem. Int. Ed. 41,
2. Solubilisation of rhPT2 in different strategies
E. coli
rhPT2 (thiol)
rhPT2 (pH)
S I S I
ER2566
Arctic Express
Chaperone modulation -
DnaK, DnaJ, GrpE, GroEL and GroES
DnaK, DnaJ, and GrpE
GroEL and GroES
Cpn10 and Cpn60
S : soluble; I: insoluble
3. Mechanism of chaperone
A B
Figure 2. Chaperone cycle of (A) GroEL and GroES, and (B) DnaK, DnaJ, and GrpE.
Results
1. Cloning of rhPT2 into pTWIN1
pTWIN1 M
Figure 1. Cloning of (A) rhPT2 (thiol) and (B) rhPT2 (pH) into pTWIN1. M is marker, 1. undigested plasmid and 2. double-digested plasmid resulting pTWIN1 and rhPT2.
A B
6000
1000 M
Table 1. rhPT2 expression at different chaperone modulation.
ArcticExpress
Expression
SDS-PAGE
The solubilisation of the rhPT2 fusion intein-tag was achieved by chaperone modulation. These results revealed that chaperones are tools for protein folding and assists the solubilisation process of rhPT2 in E. coli.
This work is supported by grant Penelitian Unggulan Strategis Nasional (PUSNAS) from Directorate of Higher Education, Ministry of National Education, Indonesia.
ArcticExpress and ER2566-chaperone co-expression successfully expressed soluble rhPT2. GroEL/GroES and Cpn60/Cpn10 are the best chaperone systems for expressing soluble rhPT2.
Size of poster is
Because of important function in clot formation thrombin has been utilised in a number of product intended for haemostasis and/or as tissue sealants. The recombinant human prethrombin-2 (rhPT2), as a thrombin precursor, is a protease-like protein which is expressed by E.
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