1. Metastasis Models Folder Title: MetModels(NoTP)
Updated: March 29, 2017

2. Shown in Presentation on Invasion and Metastases: Slides 2, 3, and 4
LiverMets Fig 2-1
Gastric adenocarcinoma,
Metastatic to the brain
(b) Colon carcinoma? Metastatic to the liver

3. Gastric to Brain Mets

4. Mammary cancer metastatic to the brain.
(Figure 2.2c, Biology of Cancer, p. 27)

5. How do we study metastases pre-clinically?
How do we determine what drives them in cell biological terms?
How to we quantitate Metastatic Potential?
How do we develop anti-metastatic strategies to test in the clinic?

6. Lung Met Treatment Model: (Metastatic rat embryo cell line) Determination of the Effectiveness of use of protease inhibitors
rTIMP = recombinant tissue-inhibitor of metallo-proteinases; metastasis suppressor function

7. Progressive Selection for Highly Metastatic Malignant Melanoma in C57Bl/6 Mice (Isaiah Fidler)

8. B16 Melanoma Metastatic to Mouse Lung Treated Placebo Treated
(Experimental Metastases by Tail-vein Injection)
Metastases Blocked by Treatment
Metastases Unblocked
Treated
Placebo Treated
p. 27
Figure 2.2a The Biology of Cancer (© Garland Science 2007)

9. Systems for Studying and Quantitating In Vivo Metastases
Metastatic Variants with High and Low Capacity to Metastasize in Mice: e.g. B16 Melanoma Variants Selected as shown in Slide 7
Challenge mice IV: “Artificial Metastases” = Don’t have to “learn” how to get into the blood-stream to get to distant sites. They are put into the blood-stream experimentally.
Challenge Mice Sub-cutaneously (SC) or
Intradermally (ID) or
Intramuscularly (IM) or
Intra-peritoneally (IP)
Measure metastases by counting tumor nodules at sites of metastases or
By Weighing Organs.
Also can do bioassay to presence of metastatic cells by growing cells from the organ with metastases in culture.

10. Systems for Visualizing In Vivo Metastases
Challenge Mice with radioactively-labelled Metastatic Cells
Use Monoclonal Antibodies with Fluorescent or radioactive reporter group:
Antibodies with Metastases markers as antigenic Targets
(See Slide 11)
Label Metastatic Cells with Fluorescent Proteins to Identify where the Metastatic Cells Have Gone
(See “Meta-Mouse” Slide 12)

11. Imaging of Metastatic Colon Carcinoma with
Radioactive-Iodine-Labelled Monoclonal Ab to A33 Ag
Colon CA A33 Monoclonal
Lloyd Old, Scientific American, August, 1996, p. 138)
Arm
Head
SeeMets

12. MetaMouse

13. Following are Three Turning Point Quiz Questions
Please put away all devices other than the NXT Transmitter.

17. Information for Exam Two: April 4, 2017
Ends Here