1. Does function explain size?
Image- the_human_body.htm
Image-

2. Does function explain size?
3.2 billion bp
250 billion bp
137 million bp
9.1 million bp
1.7 million bp
Whisk fern
Image- primer/analysis.shtml
Image- fruitflyimage.htm
Image- BioBookDiversity_5.html

3. 9.1 Mb 7.8 Mb 7.1 Mb 4.7 Mb 1.7Mb Npun Tery Avar Gvi Pro9313 The Giant
Sizes of all cyanobacteria
Images- www-cyanosite.bio.purdue.edu/ images/images.html

4. -Heterotrophic (dark w/sugar)
Npun
-Photosynthesis
-Heterocysts (fix N2 )
-Heterotrophic (dark w/sugar)
-Hormogonia
-Symbiosis
-Akinetes
Functions of Nostoc- Photosynthesis, Grow in Dark with sugar, Akinetes (spore), Heterocysts, Hormogonia (mobile, colonize), Fix N2, Symbiosis
Image oferta_m.htm
Meeks, J.C. et. al An overview of the genome of Nostoc punctiforme, a multicellular, symbiotic cyanobarcterium. Photosynthesis Research 70:

5. Functions
Closest
Share functions with other cyano-maybe

6. More functions---More genes?
Npun-7412 Avar-6809 SS
Maybe- Or Duplicates?
More families of genes?
More genes or extra stuff? Duplicates?, more genes per family, more families
More genes in each family?

7. Counting genes… 0 avar_gene1088 0 tll0876 1 Pro1503 Pattern match
20082 tsr2400
Pattern match
Avar
Total genes- 3
How solve—list Tribe? Family and genes

8. Counting families… Count family Count genes per family if count = 0 1
Avar – 2 families 1 2
0 avar_gene1088
0 tll0876
1 Pro1503
1 avar_gene713
avar_gene83 …
20082 tsr2400
Pattern match for each org and count
If at least 1 gene in family for each org. count fam.

9. More families?

10. Genes per family? total genes/ total families Graph- avg. per family
Yes…Nostoc more gene per family

11. Average -Skewed? Genes per family Take median All cyano - 1 Skew?
Collect count of genes for each family
Median for all is one.

12. Unique families? How many unique to Nostoc?
How many unique to Nostoc and one other. (share) phylog.

13. Earlier– phylogenetic trees based on 16-S RNA difference
Shared families?
Earlier– phylogenetic trees based on 16-S RNA difference
Unique to Npun and other
Maybe explain why Nostoc bigger
Do phen trees based on both– RNA from assingment (march 30 Problem set 2) (or from how many Nostoc shares)

14. More families
More genes per family
Lots of genes in a few families
Share families- shared functions?