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Response to the Comment by Ainavarapu et al.

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Presentation on theme: "Response to the Comment by Ainavarapu et al."— Presentation transcript:

1 Response to the Comment by Ainavarapu et al.
Matthias Rief, Jan Philipp Junker, Michael Schlierf, Kai Hell, Walter Neupert  Biophysical Journal  Volume 91, Issue 5, Pages (September 2006) DOI: /biophysj Copyright © 2006 The Biophysical Society Terms and Conditions

2 Figure 1 Force curves for the DHFR-Ddfilamin-protein construct. Our detection limit of 20 pN is indicated by the gray line. (a) In the vast majority of cases, DHFR unfolds at 50–80 pN after one or more Ddfilamin domains. (b) Only in very rare cases, DHFR unfolds at forces below 30 pN. Although these events are extremely rare, they are easily detectable with our assay. Biophysical Journal  , DOI: ( /biophysj ) Copyright © 2006 The Biophysical Society Terms and Conditions

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