1. Volume 114, Issue 5, Pages 1024-1034 (May 1998)
Reduced plasma cholesterol and increased fecal sterol loss in multidrug resistance gene 2 P-glycoprotein-deficient mice 
Peter J. Voshol*, Rick Havinga*, Henk Wolters*, Roel Ottenhoff‡, Hans M.G. Princen§, Ronald P.J. Oude Elferink‡, Albert K. Groen‡, Folkert Kuipers* 
Gastroenterology 
Volume 114, Issue 5, Pages (May 1998)
DOI: /S (98)
Copyright © 1998 American Gastroenterological Association Terms and Conditions

2. Fig. 1
Hepatic lipid content in (A) male and (B) female (+/+) (2) and (−/−) (■) mice on a regular chow diet. FC, free cholesterol; CE, cholesterol ester; TG, triglycerides; PL, phospholipids (value divided by 10). All values represent nanomoles per milligram of protein (n = 4; mean ± SD). *P = 0.02, Mann–Whitney U test.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

3. Fig. 1
Hepatic lipid content in (A) male and (B) female (+/+) (2) and (−/−) (■) mice on a regular chow diet. FC, free cholesterol; CE, cholesterol ester; TG, triglycerides; PL, phospholipids (value divided by 10). All values represent nanomoles per milligram of protein (n = 4; mean ± SD). *P = 0.02, Mann–Whitney U test.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

4. Fig. 2
(A) Plasma cholesterol and (B) triglyceride levels in chow-fed male and female (+/+) (2) and (−/−) (■) mice. Values represent millimoles per liter; n = 10 male (+/+), n = 8 male (−/−), n = 8 female (+/+), and n = 8 female (−/−). *P < 0.05, Mann–Whitney U test.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

5. Fig. 2
(A) Plasma cholesterol and (B) triglyceride levels in chow-fed male and female (+/+) (2) and (−/−) (■) mice. Values represent millimoles per liter; n = 10 male (+/+), n = 8 male (−/−), n = 8 female (+/+), and n = 8 female (−/−). *P < 0.05, Mann–Whitney U test.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

6. Fig. 3
(A) Cholesterol and (B) triglyceride profiles after FPLC size chromatography of plasma from male (+/+) (dotted line) and (−/−) (bold line) mice. Values represent millimoles per liter in each fraction. The mean of three samples is shown.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

7. Fig. 3
(A) Cholesterol and (B) triglyceride profiles after FPLC size chromatography of plasma from male (+/+) (dotted line) and (−/−) (bold line) mice. Values represent millimoles per liter in each fraction. The mean of three samples is shown.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

8. Fig. 4
Western blot analysis of apoB and apoA-I in FPLC fractions of plasma from male (+/+) and (−/−) mice as shown in Figure 3. Protein present in the various fractions was separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis, blotted onto nitrocellulose membranes, and visualized as described in detail in Materials and Methods.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

9. Fig. 5
Hepatic mRNA levels in livers of chow-fed male (+/+) (2) and (−/−) (■) mice. Values represent the ratio of specific mRNA to RIBO mRNA as detailed in Materials and Methods. Values for (+/+) mice were taken as 100%. 7a, cholesterol 7α-hydroxylase; 270H, cholesterol 27-hydroxylase; LDLr, LDL receptor; HMG, HMG-CoA reductase; ApoA-I, apolipoprotein A-I; ApoB, apolipoprotein B; alb, albumin; FNA, α-fibrinogen (n = 3; mean ± SD). No significant differences were observed between groups, as assessed by Mann–Whitney U test.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

10. Fig. 6. (A) Plasma cholesterol decay and (B) recovered radioactivity in neutral and acidic fractions of feces after intravenous injection of 3H-cholesterol in chow-fed control (+/+) and (−/−) mice. Data for (+/+) mice are indicated by open symbols and those for (−/−) mice by closed symbols. (A) Isotopic data are expressed as the fraction of injected radioactivity per micromoles of cholesterol. The plasma decay data were analyzed by a two-compartment model, showing the following kinetic parameters: V1 (mL) Pool size1 (μmol) V2 (mL) k10 k12 k21 (+/+) 6.3 ± ,4 ± ± ± ± ± 0.15 (−/−) 12.2 ± 3.3* 40.7 ± ± 3.7* 0.24 ± ± ± 0.14 *Significant difference between (+/+) and (−/−) shown for the 17-day point only for reasons of clarity. V1 and V2, distribution volume of first and second compartment, respectively; k10, k12, k21, elimination rate constants describing removal and transfer between pools. (B) Cumulative excretion of radioactivity in neutral sterol fraction (diamonds) and acidic sterol fraction (squares) after intravenous injection of 3H-cholesterol in (+/+) and (−/−) mice. Values represent the percentage of injected dose (mean ± SD; n = 5 per group).
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

11. Fig. 6. (A) Plasma cholesterol decay and (B) recovered radioactivity in neutral and acidic fractions of feces after intravenous injection of 3H-cholesterol in chow-fed control (+/+) and (−/−) mice. Data for (+/+) mice are indicated by open symbols and those for (−/−) mice by closed symbols. (A) Isotopic data are expressed as the fraction of injected radioactivity per micromoles of cholesterol. The plasma decay data were analyzed by a two-compartment model, showing the following kinetic parameters: V1 (mL) Pool size1 (μmol) V2 (mL) k10 k12 k21 (+/+) 6.3 ± ,4 ± ± ± ± ± 0.15 (−/−) 12.2 ± 3.3* 40.7 ± ± 3.7* 0.24 ± ± ± 0.14 *Significant difference between (+/+) and (−/−) shown for the 17-day point only for reasons of clarity. V1 and V2, distribution volume of first and second compartment, respectively; k10, k12, k21, elimination rate constants describing removal and transfer between pools. (B) Cumulative excretion of radioactivity in neutral sterol fraction (diamonds) and acidic sterol fraction (squares) after intravenous injection of 3H-cholesterol in (+/+) and (−/−) mice. Values represent the percentage of injected dose (mean ± SD; n = 5 per group).
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

12. Fig. 7
(A) Fecal neutral sterol and (B) bile salt output in male (+/+) (2) and (−/−) (■) mice. Values represent the total output in micromoles per day (n = 10 [+/+] mice and n = 8 [−/−] mice; mean ± SD). *Significant difference between groups, as assessed by Mann–Whitney U test.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

13. Fig. 7
(A) Fecal neutral sterol and (B) bile salt output in male (+/+) (2) and (−/−) (■) mice. Values represent the total output in micromoles per day (n = 10 [+/+] mice and n = 8 [−/−] mice; mean ± SD). *Significant difference between groups, as assessed by Mann–Whitney U test.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions