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Volume 94, Issue 8, Pages L67-L69 (April 2008)

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1 Volume 94, Issue 8, Pages L67-L69 (April 2008)
Refractive Index Sensing of Green Fluorescent Proteins in Living Cells Using Fluorescence Lifetime Imaging Microscopy  Henk-Jan van Manen, Paul Verkuijlen, Paul Wittendorp, Vinod Subramaniam, Timo K. van den Berg, Dirk Roos, Cees Otto  Biophysical Journal  Volume 94, Issue 8, Pages L67-L69 (April 2008) DOI: /biophysj Copyright © 2008 The Biophysical Society Terms and Conditions

2 Figure 1 Confocal fluorescence images (A and C) of GFP-Rac2/p67phox-mRFP (A) and GFP-gp91phox (C) PLB-985 cells. Corresponding bright-field images are shown in B and D. Biophysical Journal  , L67-L69DOI: ( /biophysj ) Copyright © 2008 The Biophysical Society Terms and Conditions

3 Figure 2 Fluorescence intensity (A and C) and FLIM (B and D) images of GFP-gp91phox (A and B) and GFP-Rac2/p67phox-mRFP (C and D) PLB-985 cells. The lifetime scale bar ranges from 1 to 4ns. Biophysical Journal  , L67-L69DOI: ( /biophysj ) Copyright © 2008 The Biophysical Society Terms and Conditions

4 Figure 3 Fluorescence lifetime histograms of GFP-gp91phox (blue), GFP-gp91phox/p67phox-mRFP (green), and GFP-Rac2/p67phox-mRFP (red) PLB-985 cells. Curves represent FLIM data recorded from 32 (blue), 28 (green), and 65 (red) cells. Biophysical Journal  , L67-L69DOI: ( /biophysj ) Copyright © 2008 The Biophysical Society Terms and Conditions

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