1. This Week Score Conjugation Plates,
Start High Frequency of Recombination (HFR) experiment,
Continue Nasonia experiment.

2. High Frequency of Recombination (Hfr)
...bacteria exhibiting a high frequency of recombination,
an alteration DNA sequence such that the genotype of subsequent individuals differs from the parent,
…specifically, strains with a chromosome integrated F factor that is able to mobilize and transfer part of the chromosome to the F- cell.

3. Hfr Cells ...F factor integration site,
...host (bacteria chromosome) integration site.
F factor
Bacterial Chromosome
Inserted F plasmid

4. F Pilus Attaches to F- Cell

5. Hfr DNA is Cut

6. F factor and Chromosomal DNA are Transferred

7. Recombination Requires Crossing over
Double Crossover

8. DNA not Incorporated into Chromosome are Digested

9. F factor inserts in different regions of the bacterial chromosome,
Also inserts in different orientations.

10. Origin of Replication Hfr Order of transfer strain
H thr azi ton lac pur gal his gly thi
thr thi gly his gal pur lac ton azi
lac pur gal his gly thi thr azi ton
gal pur lac ton azi thr thi gly his

11. Indicates direction of transfer.
F factor A A a A a
Hfr F-
Hfr DNA that is not incorporated in the F- strand, and DNA that has crossed out of the F- strand is digested.

12. Leading Gene: the first gene transferred is determined empirically.
F factor
A transfers first. A A
Hfr F-
A transfers last.
Hfr A F-
Leading Gene: the first gene transferred is determined empirically.

13. Hfr Order of transfer
strain
H thr azi ton lac pur gal his gly thi
thr thi gly his gal pur lac ton azi
lac pur gal his gly thi thr azi ton
gal pur lac ton azi thr thi gly his

14. E. coli Map 0 minutes is at the threonine,
100 minutes is required to transfer complete genome,