1. Biotechnological Research on Endophytic Microbes
from a tea Plant and the Parasitic Plant
Partomuan Simanjuntak
Research Center for Green Science
Fukuyama University
Research Centre for Biotehnology,
Indonesian Institute of Sciences (LIPI)

4. Collected at the Puncak area, West Java, Indonesia

5. tea plant

7. endophytic fungus
tea plant

11. biotransformed product (6)
500.00
absorbance
(+)-catechin (1)
0.00
0.00
10.00
20.00
30.00
４0.00
time (min)

15. biotransformed product (7, 39%)
500.00
biotransformed product (7, 39%)
absorbance
(-)-epicatechin (2, 2.4%)
0.00
0.00
10.00
20.00
30.00
４0.00
time (min)

17. biotransformed product (7, 53%)
200.00
biotransformed product (7, 53%)
absorbance
(-)-ECG (4)
(-)-epicatechin (2, 13%)
0.00
0.00
10.00
20.00
30.00
40.00
time (min)

18. biotransformed product (8, 43%)
200.00
biotransformed product (8, 43%)
absorbance
(-)-epigallocatechin (3, 19%)
(-)-EGCG (5)
0.00
0.00
10.00
20.00
30.00
40.00
time (min)

22. Collected at the Puncak area, West Java, Indonesia

23. (+)-catechin

24. Separation

25. a new fungus, Phomopsis sp.
Fungus H,
a new fungus, Phomopsis sp.

26. (+)-catechin

28. - The microorganism is always right, your friend and sensitive partner
Law of Applied Microbiology
- The microorganism is always right,
your friend and sensitive partner
- They are no stupid microorganism
- Microorganisms can (will) do anything
- Microorganism are smarter, wiser, more
energetic than chemist, microbiologist, etc
- If you take of your (microbial) friends, they
will take care of your future
(and you will live happily everyafter)
Law of Applied Microbiology
- The microorganism is always right,
your friend and sensitive partner
- They are no stupid microorganism
- Microorganisms can (will) do anything
- Microorganism are smarter, wiser, more
energetic than chemist, microbiologist, etc
- If you take of your (microbial) friends, they
will take care of your future
(and you will live happily everyafter)
(D. Parlman, 1979)

29. The Puncak area, West Java, Indonesia

31. ありがとう　ございます
Terima Kasih

32. Ｓｙｍｂｉｏｓｉｓ ｒｅｌａｔｉｏｎｓｈｉｐ between plant and endophytic microbes
Microbes which associates with living tissue of the plant. The type of interaction between endophyte and its host plant usually mutualism symbiosis. The plant providing nutrients for microbe, while the microbe transform and produce bioactive compounds

33. Chemical compounds of mahkota dewa,
LIPI
Chemical compounds of mahkota dewa,
Phaleria macrocarpa
Bioactive compound

34. Produksi mikroba endofit C. aeruginosa (Jepang)
Produksi mikroba endofit C. domestica/C.longa (Indonesia)