1. VUMC Soil Worm Activity Monitor
Mohd Fakhrurrazi Mohd Salleh (EE)
Suhaili Harun (EE)
Amani Rafie (CompE)
Saiful Azlan Adanan (BME)
March 31, 2005

2. Progress Turned in the proposal for hardware and software request
Ordered the computer and the capture card
Tried to convert the 1-hour video to analog-avi format
Apply the sliding window method in image processing

3. Operational Concept
Video Capturing
Image Processing

4. Video Capturing Operational Concept
Turn on power of all equipment (microscope, computer, camera
Insert 40mL of distilled water into a 6mm-diameter well
Load the capture card software
Put under microscope
Put a worm in the well using a pick
Image Processing
Separated the video into frames
using Windows Movie Maker
Using Matlab
Import into Matlab
Subsampling images
Color separation for image segmentation
Analysis of the difference between frames
Determine the velocity of the worm based on its centroid
Record video of the worm using the camera software
AVI-analog video file format to image processing

5. Image Processing Operational Concept
Load AVI-analog video into MatLab
Downsample each frame by 50%
Color Separation
Filter background noise by taking difference between frames
Detrend each data plot
Plot the components of the centroid
x-component
Determine worm centroid in each frame
y-component
Image Processing
Separated the video into frames
using Windows Movie Maker
Using Matlab
Import into Matlab
Subsampling images
Color separation for image segmentation
Analysis of the difference between frames
Determine the velocity of the worm based on its centroid
Apply FFT
Numerical and graphical results
Determine the frequency at the peak
Add both components
Determine sinusoidal cycle

6. Detrend
Detrending is the statistical or mathematical operation of removing trend from the series
It is often applied to remove a feature thought to distort or obscure the relationships of interest
In our case, the feature we are removing is the translation of the worm

7. Adding the velocity of both x and y components
Amplitude
Frequency
Calculated Frequency = 2.00 Hz

8. Frequency vs. time

9. Future Work Expecting to receive the computer and the capture card
Installing the capture card and software into computer
Process the one-hour worm video