1. Prepare a Wet Mount
CAPT Mike Hughey, MC, USNR

2. First, a Video

3. Obtain a Specimen Spatula Cotton-tipped Applicator
Obtain directly or from inside curve of speculum

4. Place on Microscope Slide
Use very small amount
Too much makes the slide too thick and unreadable

5. Add NaCl and KOH
One drop
Mix well
Two slides vs. one slide?

6. Add Coverslip Remove excess fluid so the coverslip is stable.
Heat the KOH slide?

7. Microscopic Examination
Start with low power
Rarely need to go above medium power
Focus up and down through the plane of the cells

8. Yeast
Single branch
Tangle of threads

9. Watch this video

10. Trichomonas About the same size as a white blood cell
Smaller than a vaginal epithelial cell
Violent motion

11. Watch this video

12. Bacterial Vaginosis Clue cells
Vaginal epithelial cells studded with bacteria
Smudged, granular appearance
Indistinct borders
Clue Cell
Normal Vaginal Epithelial Cell

13. Clue Cells