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The effect of alpha lipoic acid in a porcine in-stent restenosis model

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Presentation on theme: "The effect of alpha lipoic acid in a porcine in-stent restenosis model"— Presentation transcript:

1 The effect of alpha lipoic acid in a porcine in-stent restenosis model
Sang Yup Lim, MD, Eun Hui Bae, MD, Myung Ho Jeong, MD, Ju Han Kim, MD, Young Joon Hong, MD, Doo Sun Sim, MD, Yong Sook Kim, PhD, In Kyu Park, PhD, Youngkeun Ahn, MD, Sun-Jung Song, PhD, Dong Lyun Cho, PhD, Kyoung Seok Kim, MS, Jung Chaee Kang, MD  Journal of Cardiology  Volume 54, Issue 3, Pages (December 2009) DOI: /j.jjcc Copyright © 2009 Japanese College of Cardiology Terms and Conditions

2 Figure 1 Semiquantitative immunoblotting of phospho-p65, phosphorylated extracellular signal-regulated kinase (p-ERK), and phosphorylated signal transducers and activators of transcription (p-STAT)3 in porcine aortic smooth muscle cells. Densitometric analysis revealed increased protein expression of phospho-p65 and p-ERK after granulocyte-colony stimulating factor (G-CSF) stimulation, which was attenuated by pretreatment with α-lipoic acid (LA) in a dose-dependent manner. The protein expression of nuclear factor (NF)-κb and p-ERK were also attenuated by α-LA pretreatment. Journal of Cardiology  , DOI: ( /j.jjcc ) Copyright © 2009 Japanese College of Cardiology Terms and Conditions

3 Figure 2 Columns show cell growth rate with or without pretreatment of α-lipoic acid (LA) on granulocyte-colony stimulating factor (G-CSF) induced porcine aortic smooth muscle cell proliferation by MTT (3-[4,5-dimethylthiazole-2-yl] 2,5-diphenyl tetrazolium bromide) assay. The growth rate was accelerated by G-CSF and attenuated by α-LA pretreatment. *p<0.05 compared with control cells, #p<0.05 compared with G-CSF stimulated cells. Journal of Cardiology  , DOI: ( /j.jjcc ) Copyright © 2009 Japanese College of Cardiology Terms and Conditions

4 Figure 3 Methyl methacrylate (A and B) and hematoxylin and eosin (C and D) stain in the α-lipoic acid (LA) feeding study. In-stent neointimal area was smaller in the α-LA feeding group compared with the control group. (A) Control group; (B) α-LA feeding group. Magnification: 10×. α-LA feeding group shows lower inflammatory cell infiltration compared with control group. (C) Control group; (D) α-LA feeding group. Magnification: 200×. Journal of Cardiology  , DOI: ( /j.jjcc ) Copyright © 2009 Japanese College of Cardiology Terms and Conditions

5 Figure 4 Methyl methacrylate (A and B) and hematoxylin and eosin (C and D) stain in the α-lipoic acid (LA) coated stent study. In-stent neointimal area was smaller in the α-LA coated stent group compared with the control group. (A) Control group; (B) α-LA coating stent group. Magnification: 10×. The α-LA feeding group showed lower inflammatory cell infiltration compared with control group. (C) Control group; (D) α-LA coated stent group. Magnification: 200×. Journal of Cardiology  , DOI: ( /j.jjcc ) Copyright © 2009 Japanese College of Cardiology Terms and Conditions

6 Figure 5 Immunohistochemical staining for phosphorylated Akt and vascular endothelial growth factor (VEGF). (A) and (C) Control stent (bare metal stent); (B) and (D) α-lipoic acid (LA) coated stent. α-LA coated stent attenuated immunolabeling of p-Akt effectively after 28 days of stenting. Magnification: 200×. Journal of Cardiology  , DOI: ( /j.jjcc ) Copyright © 2009 Japanese College of Cardiology Terms and Conditions

7 Figure 6 Immunohistochemical staining for phosphorylated signal transducers and activators of transcription (p-STAT)-3 and proliferating cell nuclear antigen (PCNA). (A) and (C) Control stent (bare metal stent); (B) and (D) α-lipoic acid (LA) coated stent. α-LA coated stent attenuated immunolabeling of p-STAT-3 and PCNA effectively after 28 days of stenting. Magnification: 200×. Journal of Cardiology  , DOI: ( /j.jjcc ) Copyright © 2009 Japanese College of Cardiology Terms and Conditions

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